Establishment of human induced trophoblast stem-like cells from term villous cytotrophoblasts

Tao Bai, Chian-Yu Peng, Ivy Aneas, Noboru Sakabe, Daniela F. Requena, Christine Billstrand, Marcelo Nobrega, Carole Ober, Mana Parast, John A. Kessler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Human trophoblast stem cells (hTSC) can be isolated from first trimester placenta but not from term placenta. Here we demonstrate that villous cytotrophoblasts (vCTB) from term placenta can be reprogrammed into induced trophoblastic stem-like cells (iTSC) by introducing sets of transcription factors. The iTSCs express TSC markers such as GATA3, TEAD4 and ELF5, and are multipotent, validated by their differentiation into both extravillous trophoblasts (EVT) and syncytiotrophoblasts (STB) in vitro and in vivo. The iTSC can be passaged indefinitely in vitro without slowing of growth. The transcriptome profile of these cells closely resembles the profile of hTSC isolated from first trimester placentae but different from the term placental vCTB from which they originated. The ability to reprogram cells from term placenta into iTSC will allow study of early gestation events which impact placental function later in gestation, including preeclampsia and spontaneous preterm birth.

Original languageEnglish (US)
Article number102507
JournalStem Cell Research
Volume56
DOIs
StatePublished - Oct 2021

Keywords

  • Differentiation
  • Placenta
  • Reprogramming
  • Stem cell
  • Trophoblast

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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