Establishment of stable cell lines from primary human dental pulp stem cells

Elizabeth Guirado, Youbin Zhang, Anne George*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


This protocol is for the isolation of primary human dental pulp stem cells (DPSCs) from adult extracted molars and for the generation of high-titer lentivirus for in vitro infection of the DPSCs. Stable cell lines of dental pulp stem cells are generated, maintained in culture, and used for subsequent experiments.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages7
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Gene transfer techniques
  • Genetic recombination
  • Genetic transduction
  • Lentivirus
  • Somatic stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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