Estrogen receptor α signaling pathways differentially regulate gonadotropin subunit gene expression and serum follicle-stimulating hormone in the female mouse

C. Glidewell-Kenney, J. Weiss, L. A. Hurley, J. E. Levine, J. Larry Jameson

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Estrogen, acting via estrogen receptor (ER)α, regulates serum gonadotropin levels and pituitary gonadotropin subunit expression. However, the cellular pathways mediating this regulation are unknown. ERα signals through classical estrogen response element (ERE)-dependent genomic as well as nonclassical ERE-independent genomic and nongenomic pathways. Using targeted mutagenesis in mice to disrupt ERα DNA binding activity, we previously demonstrated that ERE-independent signaling is sufficient to suppress serum LH levels. In this study, we examined the relative roles of ERE-dependent and -independent estrogen signaling in estrogen regulation of LH, FSH, prolactin, and activin/inhibin subunit gene expression, pituitary LH and FSH protein content, and serum FSH levels. ERE-independent signaling was not sufficient for estrogen to induce pituitary prolactin mRNA or suppress pituitary LHβ mRNA, LH content, or serum FSH in estrogen-treated ovariectomized mice. However, ERE-independent signaling was sufficient to reduce pituitary glycoprotein hormone α-subunit, FSHβ, and activin-B mRNA expression. Together with previous serum LH results, these findings suggest ERE-independent ERα signaling suppresses serum LH via reduced secretion, not synthesis. Additionally, ERE-dependent and ERE-independent ERα pathways may distinctly regulate steps involved in the synthesis and secretion of FSH.

Original languageEnglish (US)
Pages (from-to)4168-4176
Number of pages9
JournalEndocrinology
Volume149
Issue number8
DOIs
StatePublished - Aug 1 2008

ASJC Scopus subject areas

  • Endocrinology

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