Abstract
Objective: This study aimed to evaluate the effect of cyclic ischemia-reperfusion (IR) injury on wound healing using a novel rabbit ear model. Materials and Methods: A lightweight clamp apparatus was developed for reversible occlusion of the central ear artery. Ventral ear wounds were analyzed postoperatively for epithelialization and granulation as well as gene expression after 3 consecutive days of IR cycling. Results: By postoperative day #7, ears showed no gross tissue necrosis, but histologic analysis of wounds confirmed a significant impairment in epithelial and granulation tissue gaps as well as total epithelial and granulation tissue areas (P < 0.001). Quantitative polymerase chain reaction analysis of IR wounds indicated significant up-regulation of heat shock protein-70 and downregulation of superoxide dismutase 1 relative to sham controls (P < 0.05). Conclusions: A novel rabbit ear model for the induction of subclinical, cyclic IR injury in cutaneous tissue has been developed that will serve as a valuable tool for the testing of new therapeutics.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 698-705 |
| Number of pages | 8 |
| Journal | Annals of plastic surgery |
| Volume | 72 |
| Issue number | SUPPL. 2 |
| DOIs | |
| State | Published - Jun 2014 |
Keywords
- Chronic wounds
- Ischemia
- Ischemia-reperfusion injury
- Rabbit ear
- Reperfusion injury
ASJC Scopus subject areas
- Surgery