Evaluation of electron capture dissociation for D atom localization at the peptide and protein levels after solution phase exchange

Jay P. Charlebois; Steven M. Patrie; Neil L. Kelleher

Evaluation of electron capture dissociation for D atom localization at the peptide and protein levels after solution phase exchange

Jay P. Charlebois^*, Steven M. Patrie, Neil L. Kelleher

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The electron capture dissociation (ECD) for localization of deuterium atom at peptide levels was investigated after solution phase exchange. THe deuterated samples were incubated for 4 hours at 75° C which was then cooled to sampling and deuterated protein was digested with pepsin. The digested protein was rapidly desalted with peptide trap and eluted with transfer line which was cooled by modified refrigerator. The deuterated experiments show back exchange of 40% for both ten second deuteration and maximally deuterated samples.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	387-388
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

@inproceedings{41273cbdb12b4596807bc66541ab75b6,

title = "Evaluation of electron capture dissociation for D atom localization at the peptide and protein levels after solution phase exchange",

abstract = "The electron capture dissociation (ECD) for localization of deuterium atom at peptide levels was investigated after solution phase exchange. THe deuterated samples were incubated for 4 hours at 75° C which was then cooled to sampling and deuterated protein was digested with pepsin. The digested protein was rapidly desalted with peptide trap and eluted with transfer line which was cooled by modified refrigerator. The deuterated experiments show back exchange of 40% for both ten second deuteration and maximally deuterated samples.",

author = "Charlebois, {Jay P.} and Patrie, {Steven M.} and Kelleher, {Neil L.}",

year = "2002",

month = dec,

day = "1",

language = "English (US)",

pages = "387--388",

booktitle = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Charlebois, JP, Patrie, SM & Kelleher, NL 2002, Evaluation of electron capture dissociation for D atom localization at the peptide and protein levels after solution phase exchange. in Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 387-388, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

Evaluation of electron capture dissociation for D atom localization at the peptide and protein levels after solution phase exchange. / Charlebois, Jay P.; Patrie, Steven M.; Kelleher, Neil L.
Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 387-388.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Evaluation of electron capture dissociation for D atom localization at the peptide and protein levels after solution phase exchange

AU - Charlebois, Jay P.

AU - Patrie, Steven M.

AU - Kelleher, Neil L.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The electron capture dissociation (ECD) for localization of deuterium atom at peptide levels was investigated after solution phase exchange. THe deuterated samples were incubated for 4 hours at 75° C which was then cooled to sampling and deuterated protein was digested with pepsin. The digested protein was rapidly desalted with peptide trap and eluted with transfer line which was cooled by modified refrigerator. The deuterated experiments show back exchange of 40% for both ten second deuteration and maximally deuterated samples.

AB - The electron capture dissociation (ECD) for localization of deuterium atom at peptide levels was investigated after solution phase exchange. THe deuterated samples were incubated for 4 hours at 75° C which was then cooled to sampling and deuterated protein was digested with pepsin. The digested protein was rapidly desalted with peptide trap and eluted with transfer line which was cooled by modified refrigerator. The deuterated experiments show back exchange of 40% for both ten second deuteration and maximally deuterated samples.

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M3 - Conference contribution

AN - SCOPUS:2442670562

SP - 387

EP - 388

BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Evaluation of electron capture dissociation for D atom localization at the peptide and protein levels after solution phase exchange

Abstract

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ASJC Scopus subject areas

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