Evaluation of methylphosphonates as analogs for detecting phosphate contacts in RNA-protein complexes

Dagmar Dertinger, Olke C. Uhlenbeck*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The well-studied interaction between the MS2 coat protein and its cognate hairpin was used to test the utility of the methylphosphonate linkage as a phosphate analog. A nitrocellulose filter binding assay was used to measure the change in binding affinity upon introduction of a single methylphosphonate stereoisomer at 13 different positions in the RNA hairpin. Comparing these data to the available crystal structure of the complex shows that all phosphates that are in proximity to the protein show a weaker binding affinity when substituted with a phosphorothioate and control positions show no change. However, in two cases, a methylphosphonate isomer either increased or decreased the binding affinity where no interaction can be detected in the flexibilit crystal structure. It is possible that methylphosphonate substitutions at these positions affect the structure or of the hairpin. The utility of the methylphosphonate substitution is compared to phosphate ethylation and phosphorothioate substitution experiments previously performed on the same system.

Original languageEnglish (US)
Pages (from-to)622-631
Number of pages10
JournalRNA
Volume7
Issue number4
DOIs
StatePublished - 2001

Keywords

  • 3-ethyl-1-nitrosourea (ENU)
  • Deprotection
  • Equilibrium binding constant
  • HPLC separation
  • MS2 bacteriophage
  • Phosphate modification
  • Phosphorothioate
  • RNA hairpin

ASJC Scopus subject areas

  • Molecular Biology

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