Evaluation of the presence of B-cell attractant chemokines in chronic rhinosinusitis

Monica Patadia*, Julian Dixon, David Conley, Rakesh Chandra, Anju Peters, Lydia A. Suh, Atsushi Kato, Roderick Carter, Kathleen Harris, Leslie Grammer, Robert Kern, Robert Schleimer

*Corresponding author for this work

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Background: B-cell responses may play a role in the pathogenesis of nasal polyposis via local IgA and IgE production and activation of eosinophils and mast cells. B-cell attracting chemokines may therefore have relevance in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNPs) Methods: Polyp and inferior turbinate tissues were obtained from CRSwNPs, CRS without NPs (CRSsNPs), and control patients; ELISA and reverse-transcription polymerase chain reaction were used to detect levels of protein and mRNA for selected B-cell chemokines (B-cell attracting chemokine 1 [CXCL13/BCA-1/BLC]), thymus expressed chemokine (CCL25/TECK), mucosae-associated epithelial chemokine (CCL28/MEC), stromal cell- derived factor-1alpha (CXCL12/SDF-1alpha), and selected chemokine receptor genes (CXCR4, CXCR5, and CXCR7). Results: BCA-1 and SDF-1alpha protein levels were significantly increased in polyp tissue compared with turbinate tissue from CRSsNP patients and controls (p < 0.05 and p < 0.01, respectively). Differences in TECK and MEC were not significant. For mRNA, expression of BCA-1 was significantly up-regulated in polyp tissue and levels correlated with CD20 mRNA expression. Additionally, significant up-regulation of mRNA for the SDF-1alpha receptors CXCR7 and CXCR4 was detected in polyps, while there was a trend for up-regulation of the BCA-1 receptor CXCR5. Conclusion: Elevated levels of the BCA-1 and SDF-1alpha and their receptors may account for an increased presence of B cells and their products, contributing to eosinophilic inflammation in patients with CRSwNP.

Original languageEnglish (US)
Pages (from-to)11-16
Number of pages6
JournalAmerican Journal of Rhinology and Allergy
Volume24
Issue number1
DOIs
StatePublished - Jan 2010

Keywords

  • B-cell recruitment
  • CCL25/TECK
  • CCL28/MEC
  • CXCL12/SDF-1alpha
  • CXCL13/BCA-1
  • Chemokines
  • Chronic rhinosinusitis
  • Nasal polyposis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Otorhinolaryngology

Fingerprint Dive into the research topics of 'Evaluation of the presence of B-cell attractant chemokines in chronic rhinosinusitis'. Together they form a unique fingerprint.

Cite this