TY - JOUR
T1 - Evaluation of Two HIV Rapid Diagnostic Tests in a Context of Strains' Genetic Diversity in Mali
AU - Togo, Josue
AU - Maiga, Almoustapha Issiaka
AU - Sylla, Mariam
AU - Kone, Bourahima
AU - Dolo, Oumar
AU - Traore, Fatoumata Tata
AU - Sangare, Samba Adama
AU - Maiga, Mamoudou
AU - Diallo, Souleymane
AU - Murphy, Robert
AU - Calvez, Vincent
AU - Marcelin, Anne Genevieve
N1 - Funding Information:
The authors thank Edimamel Company for the gracious donation of the HIV TRIDOT Rapid tests. We also thank Mr Youssouf Toure and Mr Amadou Keita from Gabriel Toure University Hospital for their technical help during the study and to all SEREFO staff. Research reported in this publication was supported by the Fogarty International Center of the National Institutes of Health under award number D43TW10350.
Publisher Copyright:
© 2019, Mary Ann Liebert, Inc., publishers.
PY - 2019/2
Y1 - 2019/2
N2 - The rapid diagnostic tests used in most resource-limited countries offer little specificity in the differentiation of HIV-1, HIV-2, and HIV-1 + 2. World Health Organization (WHO) does periodically evaluate rapid tests in use in the South Countries. Despite the prequalification from WHO, it is necessary at local level to conduct comparative studies between the available tests. To do this in Mali, we conducted a cross-sectional study at the University Teaching Hospital Gabriel Touré and the Research and Training Center (SEREFO) of Bamako on 172 samples from retrospective and prospective studies. The goal of this project was to compare the following rapid tests: "HIV TRI-DOT" and OnSite HIV1/2 Ab Plus Combo Rapid Test for screening HIV-1 and HIV-2 to "ImmunoComb II BiSpot" (gold standard). The sensibility and specificity of "HIV TRI-DOT" for HIV-1 detection were 100% each [95% confidence interval (CI): 87.13-100 and 95.31-100]. Its performance for HIV-2 detection was 95.24% (95% CI: 84.21-98.68) (sensibility) and 100% (95% CI: 72.25-100) (specificity). The positive and negative predictive values were, respectively, 100% (95% CI: 91.24-100) and 83.33% (95% CI: 55.20-95.30). The sensibility and specificity of OnSite HIV1/2 Ab Plus Combo Rapid Test assay performance were 100% (95% CI: 87.13-100 and 95.31-100). Overall, OnSite HIV Ab Combo Rapid Test proved to be a good test for warm countries and does not require refrigeration in these settings. Blood and its products can be used to perform the test, unlike "ImmunoComb II BiSpot" and "HIV TRI-DOT," which must be kept cold and cannot be done with whole blood.
AB - The rapid diagnostic tests used in most resource-limited countries offer little specificity in the differentiation of HIV-1, HIV-2, and HIV-1 + 2. World Health Organization (WHO) does periodically evaluate rapid tests in use in the South Countries. Despite the prequalification from WHO, it is necessary at local level to conduct comparative studies between the available tests. To do this in Mali, we conducted a cross-sectional study at the University Teaching Hospital Gabriel Touré and the Research and Training Center (SEREFO) of Bamako on 172 samples from retrospective and prospective studies. The goal of this project was to compare the following rapid tests: "HIV TRI-DOT" and OnSite HIV1/2 Ab Plus Combo Rapid Test for screening HIV-1 and HIV-2 to "ImmunoComb II BiSpot" (gold standard). The sensibility and specificity of "HIV TRI-DOT" for HIV-1 detection were 100% each [95% confidence interval (CI): 87.13-100 and 95.31-100]. Its performance for HIV-2 detection was 95.24% (95% CI: 84.21-98.68) (sensibility) and 100% (95% CI: 72.25-100) (specificity). The positive and negative predictive values were, respectively, 100% (95% CI: 91.24-100) and 83.33% (95% CI: 55.20-95.30). The sensibility and specificity of OnSite HIV1/2 Ab Plus Combo Rapid Test assay performance were 100% (95% CI: 87.13-100 and 95.31-100). Overall, OnSite HIV Ab Combo Rapid Test proved to be a good test for warm countries and does not require refrigeration in these settings. Blood and its products can be used to perform the test, unlike "ImmunoComb II BiSpot" and "HIV TRI-DOT," which must be kept cold and cannot be done with whole blood.
KW - HIV
KW - Mali
KW - West Africa
KW - rapid diagnostic test
UR - http://www.scopus.com/inward/record.url?scp=85060945562&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060945562&partnerID=8YFLogxK
U2 - 10.1089/aid.2017.0296
DO - 10.1089/aid.2017.0296
M3 - Article
C2 - 30560678
AN - SCOPUS:85060945562
SN - 0889-2229
VL - 35
SP - 145
EP - 149
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 2
ER -
