Evaluation of Two HIV Rapid Diagnostic Tests in a Context of Strains' Genetic Diversity in Mali

Josue Togo, Almoustapha Issiaka Maiga*, Mariam Sylla, Bourahima Kone, Oumar Dolo, Fatoumata Tata Traore, Samba Adama Sangare, Mamoudou Maiga, Souleymane Diallo, Robert Murphy, Vincent Calvez, Anne Genevieve Marcelin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The rapid diagnostic tests used in most resource-limited countries offer little specificity in the differentiation of HIV-1, HIV-2, and HIV-1 + 2. World Health Organization (WHO) does periodically evaluate rapid tests in use in the South Countries. Despite the prequalification from WHO, it is necessary at local level to conduct comparative studies between the available tests. To do this in Mali, we conducted a cross-sectional study at the University Teaching Hospital Gabriel Touré and the Research and Training Center (SEREFO) of Bamako on 172 samples from retrospective and prospective studies. The goal of this project was to compare the following rapid tests: "HIV TRI-DOT" and OnSite HIV1/2 Ab Plus Combo Rapid Test for screening HIV-1 and HIV-2 to "ImmunoComb II BiSpot" (gold standard). The sensibility and specificity of "HIV TRI-DOT" for HIV-1 detection were 100% each [95% confidence interval (CI): 87.13-100 and 95.31-100]. Its performance for HIV-2 detection was 95.24% (95% CI: 84.21-98.68) (sensibility) and 100% (95% CI: 72.25-100) (specificity). The positive and negative predictive values were, respectively, 100% (95% CI: 91.24-100) and 83.33% (95% CI: 55.20-95.30). The sensibility and specificity of OnSite HIV1/2 Ab Plus Combo Rapid Test assay performance were 100% (95% CI: 87.13-100 and 95.31-100). Overall, OnSite HIV Ab Combo Rapid Test proved to be a good test for warm countries and does not require refrigeration in these settings. Blood and its products can be used to perform the test, unlike "ImmunoComb II BiSpot" and "HIV TRI-DOT," which must be kept cold and cannot be done with whole blood.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalAIDS research and human retroviruses
Issue number2
StatePublished - Feb 2019


  • HIV
  • Mali
  • West Africa
  • rapid diagnostic test

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology
  • Immunology


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