Evaluation of Visceral Adipose Tissue Oxygenation by Blood Oxygen Level-Dependent MRI in Zucker Diabetic Fatty Rats

Hong Jian Shi; Yan Feng Li; Wen Jie Ji; Zhi Chun Lin; Wei Cai; Tao Chen; Bin Yuan; Xiu Long Niu; Han Ying Li; Wen Shu; Yu Ming Li; Fei Yuan; Xin Zhou; Zhuoli Zhang

doi:10.1002/oby.22191

Evaluation of Visceral Adipose Tissue Oxygenation by Blood Oxygen Level-Dependent MRI in Zucker Diabetic Fatty Rats

Hong Jian Shi, Yan Feng Li, Wen Jie Ji, Zhi Chun Lin, Wei Cai, Tao Chen, Bin Yuan, Xiu Long Niu, Han Ying Li, Wen Shu, Yu Ming Li, Fei Yuan^*, Xin Zhou, Zhuoli Zhang

^*Corresponding author for this work

Radiology

Research output: Contribution to journal › Article › peer-review

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Abstract

Objective: This study aimed to investigate the feasibility of blood oxygen level-dependent magnetic resonance imaging (BOLD-MRI) to evaluate visceral adipose tissue (VAT) oxygenation in Zucker diabetic fatty (ZDF) rats and its associations with systemic metaflammation. Methods: Five-week-old ZDF rats and Zucker lean (ZL) rats were fed a high-fat diet (HFD) for 18 weeks. A baseline BOLD-MRI scan of perirenal adipose tissue was performed after 8 weeks of HFD feeding, and then the rats were randomized to receive pioglitazone or a vehicle for the following 10 weeks. At sacrifice, BOLD-MRI scan, Hypoxyprobe-1 injection, and circulating T helper 17 (Th17), regulatory T (Treg) cells, and monocyte subtype flow cytometry analysis were performed. Results: HFD feeding led to a significant increase in VAT BOLD-MRI R2* signals (20.14 ± 0.23 per second vs. 21.53 ± 0.20 per second; P = 0.012), an indicator for decreased oxygenation. R2* signal was significantly correlated with VAT pimonidazole adduct-positive area, insulin resistance, Th17 and Treg cells, CD43 + and CD43+ + monocyte subtypes, and VAT macrophage infiltration. Pioglitazone treatment improved the insulin resistance and was associated with a delayed progression of VAT oxygenation. Conclusions: This work demonstrated the feasibility of BOLD-MRI for detecting the VAT oxygenation status in ZDF rats, and the BOLD-MRI signals were associated with insulin resistance and systemic metaflammation in ZDF rats during the development of obesity.

Original language	English (US)
Pages (from-to)	1017-1025
Number of pages	9
Journal	Obesity
Volume	26
Issue number	6
DOIs	https://doi.org/10.1002/oby.22191
State	Published - Jun 2018

ASJC Scopus subject areas

Medicine (miscellaneous)
Endocrinology, Diabetes and Metabolism
Endocrinology
Nutrition and Dietetics

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10.1002/oby.22191

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@article{34c05dd8573744efae1a390fafe72568,

title = "Evaluation of Visceral Adipose Tissue Oxygenation by Blood Oxygen Level-Dependent MRI in Zucker Diabetic Fatty Rats",

abstract = "Objective: This study aimed to investigate the feasibility of blood oxygen level-dependent magnetic resonance imaging (BOLD-MRI) to evaluate visceral adipose tissue (VAT) oxygenation in Zucker diabetic fatty (ZDF) rats and its associations with systemic metaflammation. Methods: Five-week-old ZDF rats and Zucker lean (ZL) rats were fed a high-fat diet (HFD) for 18 weeks. A baseline BOLD-MRI scan of perirenal adipose tissue was performed after 8 weeks of HFD feeding, and then the rats were randomized to receive pioglitazone or a vehicle for the following 10 weeks. At sacrifice, BOLD-MRI scan, Hypoxyprobe-1 injection, and circulating T helper 17 (Th17), regulatory T (Treg) cells, and monocyte subtype flow cytometry analysis were performed. Results: HFD feeding led to a significant increase in VAT BOLD-MRI R2* signals (20.14 ± 0.23 per second vs. 21.53 ± 0.20 per second; P = 0.012), an indicator for decreased oxygenation. R2* signal was significantly correlated with VAT pimonidazole adduct-positive area, insulin resistance, Th17 and Treg cells, CD43 + and CD43+ + monocyte subtypes, and VAT macrophage infiltration. Pioglitazone treatment improved the insulin resistance and was associated with a delayed progression of VAT oxygenation. Conclusions: This work demonstrated the feasibility of BOLD-MRI for detecting the VAT oxygenation status in ZDF rats, and the BOLD-MRI signals were associated with insulin resistance and systemic metaflammation in ZDF rats during the development of obesity.",

author = "Shi, {Hong Jian} and Li, {Yan Feng} and Ji, {Wen Jie} and Lin, {Zhi Chun} and Wei Cai and Tao Chen and Bin Yuan and Niu, {Xiu Long} and Li, {Han Ying} and Wen Shu and Li, {Yu Ming} and Fei Yuan and Xin Zhou and Zhuoli Zhang",

note = "Funding Information: Funding agencies: This work was supported by the National Natural Science Foundation of China (81570335 to XZ) and intramural research program from Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury (TJC1403 to FY). Disclosure: The authors declared no conflict of interest. Author contributions: FY, XZ, and ZZ conceived the project and revised the manuscript. HJS and YFL drafted the manuscript. HJS, YFL, WJJ, ZCL, WC, TC, BY, XLN, HYL, and WS participated in data collection and analysis and carried out experiments. YML, FY, XZ, and ZZ supervised the project and participated in data interpretation. All authors had final approval of the submitted manuscript. *Hong-Jian Shi and Yan-Feng Li contributed equally to this work. Additionaly Supporting Information may be found in the online version of this artcle. Received: 19 November 2017; Accepted: 17 March 2018; Published online 7 May 2018. doi:10.1002/oby.22191 Figure 1 Treatment and blood oxygen level-dependent magnetic resonance imaging (BOLD-MRI) scan protocol. Publisher Copyright: {\textcopyright} 2018 The Obesity Society",

year = "2018",

month = jun,

doi = "10.1002/oby.22191",

language = "English (US)",

volume = "26",

pages = "1017--1025",

journal = "Obesity",

issn = "1930-7381",

publisher = "Wiley-Blackwell",

number = "6",

}

TY - JOUR

T1 - Evaluation of Visceral Adipose Tissue Oxygenation by Blood Oxygen Level-Dependent MRI in Zucker Diabetic Fatty Rats

AU - Shi, Hong Jian

AU - Li, Yan Feng

AU - Ji, Wen Jie

AU - Lin, Zhi Chun

AU - Cai, Wei

AU - Chen, Tao

AU - Yuan, Bin

AU - Niu, Xiu Long

AU - Li, Han Ying

AU - Shu, Wen

AU - Li, Yu Ming

AU - Yuan, Fei

AU - Zhou, Xin

AU - Zhang, Zhuoli

N1 - Funding Information: Funding agencies: This work was supported by the National Natural Science Foundation of China (81570335 to XZ) and intramural research program from Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury (TJC1403 to FY). Disclosure: The authors declared no conflict of interest. Author contributions: FY, XZ, and ZZ conceived the project and revised the manuscript. HJS and YFL drafted the manuscript. HJS, YFL, WJJ, ZCL, WC, TC, BY, XLN, HYL, and WS participated in data collection and analysis and carried out experiments. YML, FY, XZ, and ZZ supervised the project and participated in data interpretation. All authors had final approval of the submitted manuscript. *Hong-Jian Shi and Yan-Feng Li contributed equally to this work. Additionaly Supporting Information may be found in the online version of this artcle. Received: 19 November 2017; Accepted: 17 March 2018; Published online 7 May 2018. doi:10.1002/oby.22191 Figure 1 Treatment and blood oxygen level-dependent magnetic resonance imaging (BOLD-MRI) scan protocol. Publisher Copyright: © 2018 The Obesity Society

PY - 2018/6

Y1 - 2018/6

N2 - Objective: This study aimed to investigate the feasibility of blood oxygen level-dependent magnetic resonance imaging (BOLD-MRI) to evaluate visceral adipose tissue (VAT) oxygenation in Zucker diabetic fatty (ZDF) rats and its associations with systemic metaflammation. Methods: Five-week-old ZDF rats and Zucker lean (ZL) rats were fed a high-fat diet (HFD) for 18 weeks. A baseline BOLD-MRI scan of perirenal adipose tissue was performed after 8 weeks of HFD feeding, and then the rats were randomized to receive pioglitazone or a vehicle for the following 10 weeks. At sacrifice, BOLD-MRI scan, Hypoxyprobe-1 injection, and circulating T helper 17 (Th17), regulatory T (Treg) cells, and monocyte subtype flow cytometry analysis were performed. Results: HFD feeding led to a significant increase in VAT BOLD-MRI R2* signals (20.14 ± 0.23 per second vs. 21.53 ± 0.20 per second; P = 0.012), an indicator for decreased oxygenation. R2* signal was significantly correlated with VAT pimonidazole adduct-positive area, insulin resistance, Th17 and Treg cells, CD43 + and CD43+ + monocyte subtypes, and VAT macrophage infiltration. Pioglitazone treatment improved the insulin resistance and was associated with a delayed progression of VAT oxygenation. Conclusions: This work demonstrated the feasibility of BOLD-MRI for detecting the VAT oxygenation status in ZDF rats, and the BOLD-MRI signals were associated with insulin resistance and systemic metaflammation in ZDF rats during the development of obesity.

AB - Objective: This study aimed to investigate the feasibility of blood oxygen level-dependent magnetic resonance imaging (BOLD-MRI) to evaluate visceral adipose tissue (VAT) oxygenation in Zucker diabetic fatty (ZDF) rats and its associations with systemic metaflammation. Methods: Five-week-old ZDF rats and Zucker lean (ZL) rats were fed a high-fat diet (HFD) for 18 weeks. A baseline BOLD-MRI scan of perirenal adipose tissue was performed after 8 weeks of HFD feeding, and then the rats were randomized to receive pioglitazone or a vehicle for the following 10 weeks. At sacrifice, BOLD-MRI scan, Hypoxyprobe-1 injection, and circulating T helper 17 (Th17), regulatory T (Treg) cells, and monocyte subtype flow cytometry analysis were performed. Results: HFD feeding led to a significant increase in VAT BOLD-MRI R2* signals (20.14 ± 0.23 per second vs. 21.53 ± 0.20 per second; P = 0.012), an indicator for decreased oxygenation. R2* signal was significantly correlated with VAT pimonidazole adduct-positive area, insulin resistance, Th17 and Treg cells, CD43 + and CD43+ + monocyte subtypes, and VAT macrophage infiltration. Pioglitazone treatment improved the insulin resistance and was associated with a delayed progression of VAT oxygenation. Conclusions: This work demonstrated the feasibility of BOLD-MRI for detecting the VAT oxygenation status in ZDF rats, and the BOLD-MRI signals were associated with insulin resistance and systemic metaflammation in ZDF rats during the development of obesity.

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EP - 1025

JO - Obesity

JF - Obesity

IS - 6

ER -

Evaluation of Visceral Adipose Tissue Oxygenation by Blood Oxygen Level-Dependent MRI in Zucker Diabetic Fatty Rats

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