Evidence against an abnormal hepatic microsomal lipid matrix as the primary genetic defect in the jaundiced Gunn rat

Peter F. Whitington*, Dennis D. Black, William Struve, Michael E. Dockter

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The congenitally jaundiced Gunn rat does not conjugate bilirubin but does conjugate bilirubin dimethyl diester. Partial defects in conjugating p-nitrophenol and demethylating aminopyrine are also evident. A proposed mechanism to explain this combination of findings is a defective microsomal membrane. To examine the 'matrix' of Gunn microsomal membranes, hepatic microsomes were isolated from Gunn (jj) and outbred Wistar (JJ) rats and were studied by electron paramagnetic resonance spectroscopy of 7-doxylstearic and 12-doxylstearic acid probes, fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene, glucose-6-phosphatase activity vs. temperature, and lipid analysis. The data indicate several factors related to lipid bilayer order do not differ in microsomes from jj and JJ.

Original languageEnglish (US)
Pages (from-to)774-778
Number of pages5
JournalBBA - Biomembranes
Volume812
Issue number3
DOIs
StatePublished - Feb 14 1985

Keywords

  • (Gunn rat)
  • Bilirubin
  • Genetic defect
  • Lipid matrix
  • UDPglucuronate bilirubinglucuronoside glucuronosyltransferase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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