Evidence concerning the clonal nature of chemically induced tumors: Phosphoglycerate kinase-1 isozyme patterns in chemically induced fibrosarcomas1, 2

F. D. Deamant, P. M. Iannaccone

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13 Scopus citations

Abstract

Subcutaneous fibrosarcomas were induced chemically in mosaic mice heterozygous at the Pgk-1 locus on the X-chromosome. The normal tissues of these animals displayed two electrophoretically distinct isozymes of phosphoglycerate kinase-1-[(PGK-1) EC 2.7.2.3]. The tumors were carefully dissected and analyzed with respect to PGK-1 isozymes. A combination method of multiple sampling of the primary tumor and establishment of tissue culture cell lines from the tumor was utilized. A total of 82 samples was studied from 10 tumors developed in 9 mice. Twenty cell lines were established from 9 tumors; 6 of the tumors were shown to contain neoplastic cells of exclusively PGK-1B activity, 3 of the tumors were shown to contain neoplastic cells of exclusively PGK-1A activity, and 1 tumor contained neoplastic cells with both PGK-1A and PGK-1B activities. The tumor with both A and B activities was hourglass shaped, and the pattern of activity from multiple samples of this tumor suggests that it was formed by the coalescence of a PGK-1A and a PGK-1B tumor. The data presented are concordant with results from chimeric animals and spontaneous tumors in humans and indicate that these tumors display the behavior of clonal growths.

Original languageEnglish (US)
Pages (from-to)145-150
Number of pages6
JournalJournal of the National Cancer Institute
Volume74
Issue number1
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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