Evidence for associations between the purinergic receptor P2X 7 (P2RX7) and toxoplasmosis

S. E. Jamieson, A. L. Peixoto-Rangel, A. C. Hargrave, L. A D Roubaix, E. J. Mui, N. R. Boulter, E. N. Miller, S. J. Fuller, J. S. Wiley, L. Castellucci, K. Boyer, R. G. Peixe, M. J. Kirisits, L. De Souza Elias, J. J. Coyne, R. Correa-Oliveira, M. Sautter, N. C. Smith, M. P. Lees, C. N. SwisherP. Heydemann, A. G. Noble, D. Patel, D. Bardo, D. Burrowes, D. McLone, N. Roizen, S. Withers, L. M G Bahia-Oliveira, R. McLeod, J. M. Blackwell

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Congenital Toxoplasma gondii infection can result in intracranial calcification, hydrocephalus and retinochoroiditis. Acquired infection is commonly associated with ocular disease. Pathology is characterized by strong proinflammatory responses. Ligation of ATP by purinergic receptor P2X 7, encoded by P2RX7, stimulates proinflammatory cytokines and can lead directly to killing of intracellular pathogens. To determine whether P2X 7 has a role in susceptibility to congenital toxoplasmosis, we examined polymorphisms at P2RX7 in 149 child/parent trios from North America. We found association (FBAT Z-scores 2.429; P0.015) between the derived C()G() allele (f0.68; OR2.06; 95% CI: 1.14-3.75) at single-nucleotide polymorphism (SNP) rs1718119 (1068TC; Thr-348-Ala), and a second synonymous variant rs1621388 in linkage disequilibrium with it, and clinical signs of disease per se. Analysis of clinical subgroups showed no association with hydrocephalus, with effect sizes for associations with retinal disease and brain calcifications enhanced (OR3.0-4.25; 0.004P0.009) when hydrocephalus was removed from the analysis. Association with toxoplasmic retinochoroiditis was replicated (FBAT Z-scores 3.089; P0.002) in a small family-based study (60 families; 68 affected offspring) of acquired infection in Brazil, where the ancestral T() allele (f0.296) at SNP rs1718119 was strongly protective (OR0.27; 95% CI: 0.09-0.80).

Original languageEnglish (US)
Pages (from-to)374-383
Number of pages10
JournalGenes and Immunity
Volume11
Issue number5
DOIs
StatePublished - Jul 2010

Keywords

  • Brazil
  • North America
  • genetic polymorphisms
  • purinergic receptor P2X7
  • toxoplasmosis

ASJC Scopus subject areas

  • Immunology
  • Genetics
  • Genetics(clinical)

Fingerprint

Dive into the research topics of 'Evidence for associations between the purinergic receptor P2X 7 (P2RX7) and toxoplasmosis'. Together they form a unique fingerprint.

Cite this