Evidence for autocrine basis of transformation in NIH-3t3 cells transfected with met/HGF receptor gene

Kulvinder S. Kochhar*, Mark E. Johnson, Olga Volpert, Anand P. Iyer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

NIH-3T3 cells transformed with met/HGF receptor gene proliferate in the absence of serum and growth factors. Immunocytochemical staining with anti-HGF antibody revealed intense HGF staining in the transfected cells. Additionally, these cells secrete bioactive HGF as evidenced by the ability of the conditioned media to stimulate met/HGF receptor phosphorylation in epithelial cells, and to promote migration of bovine adrenal capillary endothelial cells in a modified Boyden chamber assay. The migration of endothelial cells could be specifically inhibited by anti-HGF antibody but not by an irrelevant antibody. Suramin, a drug known to disrupt ligand-receptor interactions, inhibits the serum and growth-factor free proliferation, and the endogenous phosphorylation of met/HGF receptor in the transformed cells. Taken together, our data suggests an autocrine mode of transformation in NIH-3T3 cells transfected with met/HGF receptor gene.

Original languageEnglish (US)
Pages (from-to)303-313
Number of pages11
JournalGrowth Factors
Volume12
Issue number4
DOIs
StatePublished - Jan 1 1995

Keywords

  • Autocrine transformation
  • HGF
  • Met/HGF receptor
  • Receptor-tyrosine Kinase

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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