Evidence for autocrine basis of transformation in NIH-3t3 cells transfected with met/HGF receptor gene

Kulvinder S. Kochhar; Mark E. Johnson; Olga Volpert; Anand P. Iyer

doi:10.3109/08977199509028968

Evidence for autocrine basis of transformation in NIH-3t3 cells transfected with met/HGF receptor gene

Kulvinder S. Kochhar^*, Mark E. Johnson, Olga Volpert, Anand P. Iyer

^*Corresponding author for this work

Urology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

NIH-3T3 cells transformed with met/HGF receptor gene proliferate in the absence of serum and growth factors. Immunocytochemical staining with anti-HGF antibody revealed intense HGF staining in the transfected cells. Additionally, these cells secrete bioactive HGF as evidenced by the ability of the conditioned media to stimulate met/HGF receptor phosphorylation in epithelial cells, and to promote migration of bovine adrenal capillary endothelial cells in a modified Boyden chamber assay. The migration of endothelial cells could be specifically inhibited by anti-HGF antibody but not by an irrelevant antibody. Suramin, a drug known to disrupt ligand-receptor interactions, inhibits the serum and growth-factor free proliferation, and the endogenous phosphorylation of met/HGF receptor in the transformed cells. Taken together, our data suggests an autocrine mode of transformation in NIH-3T3 cells transfected with met/HGF receptor gene.

Original language	English (US)
Pages (from-to)	303-313
Number of pages	11
Journal	Growth Factors
Volume	12
Issue number	4
DOIs	https://doi.org/10.3109/08977199509028968
State	Published - 1995

Keywords

Autocrine transformation
HGF
Met/HGF receptor
Receptor-tyrosine Kinase

ASJC Scopus subject areas

Endocrinology
Clinical Biochemistry
Cell Biology

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@article{cbbd1f15ea0d43c397f3d8a836425389,

title = "Evidence for autocrine basis of transformation in NIH-3t3 cells transfected with met/HGF receptor gene",

abstract = "NIH-3T3 cells transformed with met/HGF receptor gene proliferate in the absence of serum and growth factors. Immunocytochemical staining with anti-HGF antibody revealed intense HGF staining in the transfected cells. Additionally, these cells secrete bioactive HGF as evidenced by the ability of the conditioned media to stimulate met/HGF receptor phosphorylation in epithelial cells, and to promote migration of bovine adrenal capillary endothelial cells in a modified Boyden chamber assay. The migration of endothelial cells could be specifically inhibited by anti-HGF antibody but not by an irrelevant antibody. Suramin, a drug known to disrupt ligand-receptor interactions, inhibits the serum and growth-factor free proliferation, and the endogenous phosphorylation of met/HGF receptor in the transformed cells. Taken together, our data suggests an autocrine mode of transformation in NIH-3T3 cells transfected with met/HGF receptor gene.",

keywords = "Autocrine transformation, HGF, Met/HGF receptor, Receptor-tyrosine Kinase",

author = "Kochhar, {Kulvinder S.} and Johnson, {Mark E.} and Olga Volpert and Iyer, {Anand P.}",

note = "Funding Information: The work was supported by grants from the Leukemia Research Foundation (K. S. K. and A. P. I.), and ACS-IL IRG-195 (K.S.K.). The authors are grateful to Dr George Vande Woude and Ms Marianne Oskarsson for SP260 anti-met antibody, and Dr Toshikazu Nakamura for HGF and anti-HGF antibodies.",

year = "1995",

doi = "10.3109/08977199509028968",

language = "English (US)",

volume = "12",

pages = "303--313",

journal = "Growth Factors",

issn = "0897-7194",

publisher = "Informa Healthcare",

number = "4",

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TY - JOUR

T1 - Evidence for autocrine basis of transformation in NIH-3t3 cells transfected with met/HGF receptor gene

AU - Kochhar, Kulvinder S.

AU - Johnson, Mark E.

AU - Volpert, Olga

AU - Iyer, Anand P.

N1 - Funding Information: The work was supported by grants from the Leukemia Research Foundation (K. S. K. and A. P. I.), and ACS-IL IRG-195 (K.S.K.). The authors are grateful to Dr George Vande Woude and Ms Marianne Oskarsson for SP260 anti-met antibody, and Dr Toshikazu Nakamura for HGF and anti-HGF antibodies.

PY - 1995

Y1 - 1995

N2 - NIH-3T3 cells transformed with met/HGF receptor gene proliferate in the absence of serum and growth factors. Immunocytochemical staining with anti-HGF antibody revealed intense HGF staining in the transfected cells. Additionally, these cells secrete bioactive HGF as evidenced by the ability of the conditioned media to stimulate met/HGF receptor phosphorylation in epithelial cells, and to promote migration of bovine adrenal capillary endothelial cells in a modified Boyden chamber assay. The migration of endothelial cells could be specifically inhibited by anti-HGF antibody but not by an irrelevant antibody. Suramin, a drug known to disrupt ligand-receptor interactions, inhibits the serum and growth-factor free proliferation, and the endogenous phosphorylation of met/HGF receptor in the transformed cells. Taken together, our data suggests an autocrine mode of transformation in NIH-3T3 cells transfected with met/HGF receptor gene.

AB - NIH-3T3 cells transformed with met/HGF receptor gene proliferate in the absence of serum and growth factors. Immunocytochemical staining with anti-HGF antibody revealed intense HGF staining in the transfected cells. Additionally, these cells secrete bioactive HGF as evidenced by the ability of the conditioned media to stimulate met/HGF receptor phosphorylation in epithelial cells, and to promote migration of bovine adrenal capillary endothelial cells in a modified Boyden chamber assay. The migration of endothelial cells could be specifically inhibited by anti-HGF antibody but not by an irrelevant antibody. Suramin, a drug known to disrupt ligand-receptor interactions, inhibits the serum and growth-factor free proliferation, and the endogenous phosphorylation of met/HGF receptor in the transformed cells. Taken together, our data suggests an autocrine mode of transformation in NIH-3T3 cells transfected with met/HGF receptor gene.

KW - Autocrine transformation

KW - HGF

KW - Met/HGF receptor

KW - Receptor-tyrosine Kinase

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Evidence for autocrine basis of transformation in NIH-3t3 cells transfected with met/HGF receptor gene

Abstract

Keywords

ASJC Scopus subject areas

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