Evidence for extrarenal production of 1α,25-dihydroxyvitamin D in man

P. W. Lambert, P. H. Stern, R. C. Avioli, N. C. Brackett, R. T. Turner, A. Greene, I. Y. Fu, N. H. Bell

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Abstract

Recent studies provide evidence for extrarenal production of 1α,25-dihydroxyvitamin D [1α,25(OH)2D]. To investigate this possibility, serum vitamin D, 25-hydroxyvitamin D (25-OHD), 24,25-dihydroxyvitamin D [24,25(OH)2D], and 1α,25(OH)2D were measured in eight adult anephric subjects. All were undergoing hemodialysis and three of them were receiving vitamin D, 50,000 or 100,000 U/d. Serum vitamin D was elevated in two of the patients given vitamin D and was abnormally low in the others. Mean serum 25-OHD was increased in patients given vitamin D (94.0 ± 7.6 ng/ml) and was normal in the others (16.4 ± 0.9 ng/ml, P<0.001). Mean serum 24,25(OH)2D was normal in patients given vitamin D (1.38 ± 0.27 ng/ml) and was low in the others (0.25 ± 0.08 ng/ml, P<0.001). Serum 24,25(OH)2D correlated significantly with serum 25-OHD (r=0.848, P<0.01). Mean serum 1α,25(OH)2D determined by receptor assay was 5.8 ± 1.9 pg/ml in patients who were not given vitamin D and was 14.1 ± 0.6 in those who were given vitamin D (P<0.001). Serum 1α,25(OH)2D correlated significantly with serum 25-OHD (r=0.911, P<0.01). Mean serum 1α,25(OH)2D, measured by bioassay, was 8.3 ± 1.9 pg/ml in patients who were not given vitamin D and was 15.9 ± 2.4 pg/ml in those who were given vitamin D (P<0.05). There was a significant correlation between the values for serum 1α,25(OH)2D obtained with the two methods (r=0.728, P<0.01). The results (a) provide evidence in man for extrarenal production of both 24,25(OH)2D and, by two independent assays, of 1α,25(OH)2D, and (b) indicate that serum values of the two dihydroxy metabolites of vitamin D in anephric subjects vary with the serum concentration of the precursor 25-OHD.

Original languageEnglish (US)
Pages (from-to)722-725
Number of pages4
JournalUnknown Journal
Volume69
Issue number3
DOIs
StatePublished - Jan 1 1982

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Vitamin D
Serum
Assays
1,25-dihydroxyvitamin D
Dihydroxycholecalciferols
Bioassay
Metabolites
Biological Assay
Renal Dialysis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Lambert, P. W., Stern, P. H., Avioli, R. C., Brackett, N. C., Turner, R. T., Greene, A., ... Bell, N. H. (1982). Evidence for extrarenal production of 1α,25-dihydroxyvitamin D in man. Unknown Journal, 69(3), 722-725. https://doi.org/10.1172/JCI110501
Lambert, P. W. ; Stern, P. H. ; Avioli, R. C. ; Brackett, N. C. ; Turner, R. T. ; Greene, A. ; Fu, I. Y. ; Bell, N. H. / Evidence for extrarenal production of 1α,25-dihydroxyvitamin D in man. In: Unknown Journal. 1982 ; Vol. 69, No. 3. pp. 722-725.
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abstract = "Recent studies provide evidence for extrarenal production of 1α,25-dihydroxyvitamin D [1α,25(OH)2D]. To investigate this possibility, serum vitamin D, 25-hydroxyvitamin D (25-OHD), 24,25-dihydroxyvitamin D [24,25(OH)2D], and 1α,25(OH)2D were measured in eight adult anephric subjects. All were undergoing hemodialysis and three of them were receiving vitamin D, 50,000 or 100,000 U/d. Serum vitamin D was elevated in two of the patients given vitamin D and was abnormally low in the others. Mean serum 25-OHD was increased in patients given vitamin D (94.0 ± 7.6 ng/ml) and was normal in the others (16.4 ± 0.9 ng/ml, P<0.001). Mean serum 24,25(OH)2D was normal in patients given vitamin D (1.38 ± 0.27 ng/ml) and was low in the others (0.25 ± 0.08 ng/ml, P<0.001). Serum 24,25(OH)2D correlated significantly with serum 25-OHD (r=0.848, P<0.01). Mean serum 1α,25(OH)2D determined by receptor assay was 5.8 ± 1.9 pg/ml in patients who were not given vitamin D and was 14.1 ± 0.6 in those who were given vitamin D (P<0.001). Serum 1α,25(OH)2D correlated significantly with serum 25-OHD (r=0.911, P<0.01). Mean serum 1α,25(OH)2D, measured by bioassay, was 8.3 ± 1.9 pg/ml in patients who were not given vitamin D and was 15.9 ± 2.4 pg/ml in those who were given vitamin D (P<0.05). There was a significant correlation between the values for serum 1α,25(OH)2D obtained with the two methods (r=0.728, P<0.01). The results (a) provide evidence in man for extrarenal production of both 24,25(OH)2D and, by two independent assays, of 1α,25(OH)2D, and (b) indicate that serum values of the two dihydroxy metabolites of vitamin D in anephric subjects vary with the serum concentration of the precursor 25-OHD.",
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Lambert, PW, Stern, PH, Avioli, RC, Brackett, NC, Turner, RT, Greene, A, Fu, IY & Bell, NH 1982, 'Evidence for extrarenal production of 1α,25-dihydroxyvitamin D in man', Unknown Journal, vol. 69, no. 3, pp. 722-725. https://doi.org/10.1172/JCI110501

Evidence for extrarenal production of 1α,25-dihydroxyvitamin D in man. / Lambert, P. W.; Stern, P. H.; Avioli, R. C.; Brackett, N. C.; Turner, R. T.; Greene, A.; Fu, I. Y.; Bell, N. H.

In: Unknown Journal, Vol. 69, No. 3, 01.01.1982, p. 722-725.

Research output: Contribution to journalArticle

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AU - Lambert, P. W.

AU - Stern, P. H.

AU - Avioli, R. C.

AU - Brackett, N. C.

AU - Turner, R. T.

AU - Greene, A.

AU - Fu, I. Y.

AU - Bell, N. H.

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N2 - Recent studies provide evidence for extrarenal production of 1α,25-dihydroxyvitamin D [1α,25(OH)2D]. To investigate this possibility, serum vitamin D, 25-hydroxyvitamin D (25-OHD), 24,25-dihydroxyvitamin D [24,25(OH)2D], and 1α,25(OH)2D were measured in eight adult anephric subjects. All were undergoing hemodialysis and three of them were receiving vitamin D, 50,000 or 100,000 U/d. Serum vitamin D was elevated in two of the patients given vitamin D and was abnormally low in the others. Mean serum 25-OHD was increased in patients given vitamin D (94.0 ± 7.6 ng/ml) and was normal in the others (16.4 ± 0.9 ng/ml, P<0.001). Mean serum 24,25(OH)2D was normal in patients given vitamin D (1.38 ± 0.27 ng/ml) and was low in the others (0.25 ± 0.08 ng/ml, P<0.001). Serum 24,25(OH)2D correlated significantly with serum 25-OHD (r=0.848, P<0.01). Mean serum 1α,25(OH)2D determined by receptor assay was 5.8 ± 1.9 pg/ml in patients who were not given vitamin D and was 14.1 ± 0.6 in those who were given vitamin D (P<0.001). Serum 1α,25(OH)2D correlated significantly with serum 25-OHD (r=0.911, P<0.01). Mean serum 1α,25(OH)2D, measured by bioassay, was 8.3 ± 1.9 pg/ml in patients who were not given vitamin D and was 15.9 ± 2.4 pg/ml in those who were given vitamin D (P<0.05). There was a significant correlation between the values for serum 1α,25(OH)2D obtained with the two methods (r=0.728, P<0.01). The results (a) provide evidence in man for extrarenal production of both 24,25(OH)2D and, by two independent assays, of 1α,25(OH)2D, and (b) indicate that serum values of the two dihydroxy metabolites of vitamin D in anephric subjects vary with the serum concentration of the precursor 25-OHD.

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Lambert PW, Stern PH, Avioli RC, Brackett NC, Turner RT, Greene A et al. Evidence for extrarenal production of 1α,25-dihydroxyvitamin D in man. Unknown Journal. 1982 Jan 1;69(3):722-725. https://doi.org/10.1172/JCI110501