Evidence for N coordination to Fe in the [2Fe-2S] center in yeast mitochondrial complex III Comparison with similar findings for analogous bacterial [2Fe-2S] proteins

Joshua Telser; Brian M. Hoffman; Russell LoBrutto; Tomoko Ohnishi; Ah Lim Tsai; David Simpkin; Graham Palmer

doi:10.1016/0014-5793(87)80024-8

Evidence for N coordination to Fe in the [2Fe-2S] center in yeast mitochondrial complex III Comparison with similar findings for analogous bacterial [2Fe-2S] proteins

Joshua Telser^*, Brian M. Hoffman, Russell LoBrutto, Tomoko Ohnishi, Ah Lim Tsai, David Simpkin, Graham Palmer

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

Yeast mitochondrial complex III contains a subunit with a [2Fe-2S] cluster (the Rieske center) that has unusual physical and chemical properties. For apparently similar centers isolated from bacteria, it has been shown by electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) measurements that these [2Fe-2S] centers are coordinated by at least one and probably two nitrogen ligands. This work describes similar ENDOR and ESEEM studies on the intact mitochondrial complex. We find that this [2Fe-2S] cluster exhibits ESEEM and ENDOR properties that appear to be indistinguishable from those observed with the isolated bacterial systems. Furthermore, changes in EPR lineshape that occur as complex III is progressively reduced are not accompanied by any changes in the nitrogen coupling parameters. This spectroscopic evidence for nitrogen coordination is supported by published sequence data on four Rieske iron-sulfur subunits. It seems likely that this is a general characteristic of such [2Fe-2S] redox active centers.

Original language	English (US)
Pages (from-to)	117-121
Number of pages	5
Journal	FEBS Letters
Volume	214
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)80024-8
State	Published - Apr 6 1987

Funding

This work was supportedb y National Intitutes of Health grantsH L 13531( B.M.H.), GM 23371 (G.P.), and GM 25052( to J. Leigh), the Robert E. Welch Foundation C-636 (G.P.), National Science Foundation grant DMB 8606575( B.M.H.), and NRSA a.wardG M 10604-02(J .T.).

Keywords

Complex III
ENDOR
ESEEM
Iron-sulfur cluster
Mitochondria

ASJC Scopus subject areas

Genetics
Molecular Biology
Biophysics
Structural Biology
Biochemistry
Cell Biology

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10.1016/0014-5793(87)80024-8

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title = "Evidence for N coordination to Fe in the [2Fe-2S] center in yeast mitochondrial complex III Comparison with similar findings for analogous bacterial [2Fe-2S] proteins",

abstract = "Yeast mitochondrial complex III contains a subunit with a [2Fe-2S] cluster (the Rieske center) that has unusual physical and chemical properties. For apparently similar centers isolated from bacteria, it has been shown by electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) measurements that these [2Fe-2S] centers are coordinated by at least one and probably two nitrogen ligands. This work describes similar ENDOR and ESEEM studies on the intact mitochondrial complex. We find that this [2Fe-2S] cluster exhibits ESEEM and ENDOR properties that appear to be indistinguishable from those observed with the isolated bacterial systems. Furthermore, changes in EPR lineshape that occur as complex III is progressively reduced are not accompanied by any changes in the nitrogen coupling parameters. This spectroscopic evidence for nitrogen coordination is supported by published sequence data on four Rieske iron-sulfur subunits. It seems likely that this is a general characteristic of such [2Fe-2S] redox active centers.",

keywords = "Complex III, ENDOR, ESEEM, Iron-sulfur cluster, Mitochondria",

author = "Joshua Telser and Hoffman, {Brian M.} and Russell LoBrutto and Tomoko Ohnishi and Tsai, {Ah Lim} and David Simpkin and Graham Palmer",

note = "Funding Information: This work was supportedb y National Intitutes of Health grantsH L 13531( B.M.H.), GM 23371 (G.P.), and GM 25052( to J. Leigh), the Robert E. Welch Foundation C-636 (G.P.), National Science Foundation grant DMB 8606575( B.M.H.), and NRSA a.wardG M 10604-02(J .T.).",

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language = "English (US)",

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AU - Telser, Joshua

AU - Hoffman, Brian M.

AU - LoBrutto, Russell

AU - Ohnishi, Tomoko

AU - Tsai, Ah Lim

AU - Simpkin, David

AU - Palmer, Graham

N1 - Funding Information: This work was supportedb y National Intitutes of Health grantsH L 13531( B.M.H.), GM 23371 (G.P.), and GM 25052( to J. Leigh), the Robert E. Welch Foundation C-636 (G.P.), National Science Foundation grant DMB 8606575( B.M.H.), and NRSA a.wardG M 10604-02(J .T.).

PY - 1987/4/6

Y1 - 1987/4/6

N2 - Yeast mitochondrial complex III contains a subunit with a [2Fe-2S] cluster (the Rieske center) that has unusual physical and chemical properties. For apparently similar centers isolated from bacteria, it has been shown by electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) measurements that these [2Fe-2S] centers are coordinated by at least one and probably two nitrogen ligands. This work describes similar ENDOR and ESEEM studies on the intact mitochondrial complex. We find that this [2Fe-2S] cluster exhibits ESEEM and ENDOR properties that appear to be indistinguishable from those observed with the isolated bacterial systems. Furthermore, changes in EPR lineshape that occur as complex III is progressively reduced are not accompanied by any changes in the nitrogen coupling parameters. This spectroscopic evidence for nitrogen coordination is supported by published sequence data on four Rieske iron-sulfur subunits. It seems likely that this is a general characteristic of such [2Fe-2S] redox active centers.

AB - Yeast mitochondrial complex III contains a subunit with a [2Fe-2S] cluster (the Rieske center) that has unusual physical and chemical properties. For apparently similar centers isolated from bacteria, it has been shown by electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) measurements that these [2Fe-2S] centers are coordinated by at least one and probably two nitrogen ligands. This work describes similar ENDOR and ESEEM studies on the intact mitochondrial complex. We find that this [2Fe-2S] cluster exhibits ESEEM and ENDOR properties that appear to be indistinguishable from those observed with the isolated bacterial systems. Furthermore, changes in EPR lineshape that occur as complex III is progressively reduced are not accompanied by any changes in the nitrogen coupling parameters. This spectroscopic evidence for nitrogen coordination is supported by published sequence data on four Rieske iron-sulfur subunits. It seems likely that this is a general characteristic of such [2Fe-2S] redox active centers.

KW - Complex III

KW - ENDOR

KW - ESEEM

KW - Iron-sulfur cluster

KW - Mitochondria

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Evidence for N coordination to Fe in the [2Fe-2S] center in yeast mitochondrial complex III Comparison with similar findings for analogous bacterial [2Fe-2S] proteins

Abstract

Funding

Keywords

ASJC Scopus subject areas

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