Evidence for N coordination to Fe in the [2Fe-2S] center in yeast mitochondrial complex III Comparison with similar findings for analogous bacterial [2Fe-2S] proteins

Joshua Telser*, Brian M. Hoffman, Russell LoBrutto, Tomoko Ohnishi, Ah Lim Tsai, David Simpkin, Graham Palmer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Yeast mitochondrial complex III contains a subunit with a [2Fe-2S] cluster (the Rieske center) that has unusual physical and chemical properties. For apparently similar centers isolated from bacteria, it has been shown by electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM) measurements that these [2Fe-2S] centers are coordinated by at least one and probably two nitrogen ligands. This work describes similar ENDOR and ESEEM studies on the intact mitochondrial complex. We find that this [2Fe-2S] cluster exhibits ESEEM and ENDOR properties that appear to be indistinguishable from those observed with the isolated bacterial systems. Furthermore, changes in EPR lineshape that occur as complex III is progressively reduced are not accompanied by any changes in the nitrogen coupling parameters. This spectroscopic evidence for nitrogen coordination is supported by published sequence data on four Rieske iron-sulfur subunits. It seems likely that this is a general characteristic of such [2Fe-2S] redox active centers.

Original languageEnglish (US)
Pages (from-to)117-121
Number of pages5
JournalFEBS Letters
Volume214
Issue number1
DOIs
StatePublished - Apr 6 1987

Keywords

  • Complex III
  • ENDOR
  • ESEEM
  • Iron-sulfur cluster
  • Mitochondria

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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