Evidence for the participation of the neuron-specific CDK5 activator p35 during laminin-enhanced axonal growth

Gabriela Paglini, Gustavo Pigino, Patricia Kunda, Gerardo Morfini, Ricardo Maccioni, Santiago Quiroga, Adriana Ferreira, Alfredo Cáceres*

*Corresponding author for this work

Research output: Contribution to journalArticle

163 Scopus citations

Abstract

Cultures of cerebellar macroneurons were used to study the pattern of expression, subcellular localization, and function of the neuronal cdk5 activator p35 during laminin-enhanced axonal growth. The results obtained indicate that laminin, an extracellular matrix molecule capable of selectively stimulating axonal extension and promoting MAP1B phosphorylation at a proline-directed protein kinase epitope, selectively stimulates p35 expression, increases its association with the subcortical cytoskeleton, and accelerates its redistribution to the axonal growth cones. Besides, suppression of p35, but not of a highly related isoform designated as p39, by antisense oligonucleotide treatment selectively reduces cdk5 activity, laminin-enhanced axonal elongation, and MAP1b phosphorylation. Taken collectively, the present results suggest that cdk5/p35 may serve as an important regulatory linker between environmental signals (e.g., laminin) and constituents of the intracellular machinery (e.g., MAP1B) involved in axonal elongation.

Original languageEnglish (US)
Pages (from-to)9858-9869
Number of pages12
JournalJournal of Neuroscience
Volume18
Issue number23
StatePublished - Dec 1 1998

Keywords

  • Antisense oligonucleotides
  • Axon
  • Development
  • Growth cones
  • MAP1b
  • Neuronal cultures
  • Neurons
  • Phosphorylation
  • cdk5
  • p35

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Evidence for the participation of the neuron-specific CDK5 activator p35 during laminin-enhanced axonal growth'. Together they form a unique fingerprint.

  • Cite this

    Paglini, G., Pigino, G., Kunda, P., Morfini, G., Maccioni, R., Quiroga, S., Ferreira, A., & Cáceres, A. (1998). Evidence for the participation of the neuron-specific CDK5 activator p35 during laminin-enhanced axonal growth. Journal of Neuroscience, 18(23), 9858-9869.