Evidence for the Role of Solvated Metal Hydroxide in the Hammerhead Cleavage Mechanism

S. C. Dahm; W. B. Derrick; O. C. Uhlenbeck

doi:10.1021/bi00211a013

Evidence for the Role of Solvated Metal Hydroxide in the Hammerhead Cleavage Mechanism

S. C. Dahm, W. B. Derrick, O. C. Uhlenbeck^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

318 Scopus citations

Abstract

The log of the rate of the chemical step of hammerhead cleavage in Mg²⁺ increases linearly with pH between pH 5.7 and 8.9. A slope of approximately 1 indicates that a single deprotonation is required for cleavage. Hammerhead pH-rate profiles with Ca²⁺, Mn²⁺, Co²⁺, and Cd²⁺ correlate well with the pK_a's of these ions in water. This relationship between the pK_a's and the pH-rate profile suggests that a metal hydroxide bound to the hammerhead RNA acts as the base in the cleavage mechanism.

Original language	English (US)
Pages (from-to)	13040-13045
Number of pages	6
Journal	Biochemistry
Volume	32
Issue number	48
DOIs	https://doi.org/10.1021/bi00211a013
State	Published - 1993

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ASJC Scopus subject areas

Biochemistry

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abstract = "The log of the rate of the chemical step of hammerhead cleavage in Mg2+ increases linearly with pH between pH 5.7 and 8.9. A slope of approximately 1 indicates that a single deprotonation is required for cleavage. Hammerhead pH-rate profiles with Ca2+, Mn2+, Co2+, and Cd2+ correlate well with the pKa's of these ions in water. This relationship between the pKa's and the pH-rate profile suggests that a metal hydroxide bound to the hammerhead RNA acts as the base in the cleavage mechanism.",

author = "Dahm, {S. C.} and Derrick, {W. B.} and Uhlenbeck, {O. C.}",

year = "1993",

doi = "10.1021/bi00211a013",

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volume = "32",

pages = "13040--13045",

journal = "Biochemistry",

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T1 - Evidence for the Role of Solvated Metal Hydroxide in the Hammerhead Cleavage Mechanism

AU - Dahm, S. C.

AU - Derrick, W. B.

AU - Uhlenbeck, O. C.

PY - 1993

Y1 - 1993

N2 - The log of the rate of the chemical step of hammerhead cleavage in Mg2+ increases linearly with pH between pH 5.7 and 8.9. A slope of approximately 1 indicates that a single deprotonation is required for cleavage. Hammerhead pH-rate profiles with Ca2+, Mn2+, Co2+, and Cd2+ correlate well with the pKa's of these ions in water. This relationship between the pKa's and the pH-rate profile suggests that a metal hydroxide bound to the hammerhead RNA acts as the base in the cleavage mechanism.

AB - The log of the rate of the chemical step of hammerhead cleavage in Mg2+ increases linearly with pH between pH 5.7 and 8.9. A slope of approximately 1 indicates that a single deprotonation is required for cleavage. Hammerhead pH-rate profiles with Ca2+, Mn2+, Co2+, and Cd2+ correlate well with the pKa's of these ions in water. This relationship between the pKa's and the pH-rate profile suggests that a metal hydroxide bound to the hammerhead RNA acts as the base in the cleavage mechanism.

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JO - Biochemistry

JF - Biochemistry

IS - 48

ER -

Evidence for the Role of Solvated Metal Hydroxide in the Hammerhead Cleavage Mechanism

Abstract

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