Evidence for the Role of Solvated Metal Hydroxide in the Hammerhead Cleavage Mechanism

S. C. Dahm, W. B. Derrick, O. C. Uhlenbeck*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

309 Scopus citations

Abstract

The log of the rate of the chemical step of hammerhead cleavage in Mg2+ increases linearly with pH between pH 5.7 and 8.9. A slope of approximately 1 indicates that a single deprotonation is required for cleavage. Hammerhead pH-rate profiles with Ca2+, Mn2+, Co2+, and Cd2+ correlate well with the pKa's of these ions in water. This relationship between the pKa's and the pH-rate profile suggests that a metal hydroxide bound to the hammerhead RNA acts as the base in the cleavage mechanism.

Original languageEnglish (US)
Pages (from-to)13040-13045
Number of pages6
JournalBiochemistry
Volume32
Issue number48
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Biochemistry

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