Evidence that oncostatin M synergizes with IL-4 signaling to induce TSLP expression in chronic rhinosinusitis with nasal polyps

Bao Feng Wang, Ping Ping Cao*, James E. Norton, Julie A. Poposki, Aiko I. Klingler, Lydia A. Suh, Roderick Carter, Julia H. Huang, Junqin Bai, Whitney W. Stevens, Bruce K. Tan, Anju T. Peters, Leslie C. Grammer, David B. Conley, Kevin C. Welch, Zheng Liu, Robert C. Kern, Atsushi Kato, Robert P. Schleimer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background: Oncostatin M (OSM) may promote type 2 inflammation in chronic rhinosinusitis with nasal polyps (CRSwNP) by inducing thymic stromal lymphopoietin (TSLP). Objective: We sought to study the impact of OSM on TSLP synthesis and release from nasal epithelial cells (NECs). Methods: OSM receptors, IL-4 receptors (IL-4R), and TSLP were evaluated in mucosal tissue and primary NECs from patients with CRSwNP by quantitative PCR and immunofluorescence. Air-liquid interface–cultured NECs were stimulated with cytokines, including OSM, and quantitative PCR, ELISA, Western blot, and flow cytometry were used to assess the expression of OSM receptors, IL-4R, and TSLP. Results: Increased levels of OSM receptor β chain (OSMRβ), IL-4Rα, and TSLP were observed in nasal polyp tissues and primary epithelial cells from nasal polyps of patients with CRSwNP compared with control tissues or cells from control subjects. The level of expression of OSMRβ in tissue was correlated with levels of both IL-4Rα and TSLP. OSM stimulation of NECs increased the expression of OSMRβ and IL-4Rα. Stimulation with IL-4 plus OSM augmented the production of TSLP; the response was suppressed by a signal transducer and activator of transcription 6 inhibitor. Stimulation of NECs with IL-4 plus OSM increased the expression of proprotein convertase subtilisin/kexin 3, an enzyme that truncates and activates TSLP. Conclusions: OSM increases the expression of IL-4Rα and synergizes with IL-4 to induce the synthesis and release of TSLP in NECs. Because the combination of IL-4 and OSM also augmented the expression of proprotein convertase subtilisin/kexin 3, these results suggest that OSM can induce both synthesis and posttranslational processing/activation of TSLP, promoting type 2 inflammation.

Original languageEnglish (US)
Pages (from-to)1379-1390.e11
JournalJournal of Allergy and Clinical Immunology
Volume151
Issue number5
DOIs
StatePublished - May 2023

Keywords

  • IL-4Rα
  • OSM
  • OSMRβ
  • TSLP
  • chronic rhinosinusitis
  • nasal epithelial cells
  • nasal polyps
  • type 2–dominant inflammation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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