Evolutionary tuning impacts the design of bacterial tRNAs for the incorporation of unnatural amino acids by ribosomes

Olke C. Uhlenbeck, Jared M. Schrader

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

In order to function on the ribosome with uniform rate and adequate accuracy, each bacterial tRNA has evolved to have a characteristic sequence and set of modifications that compensate for the differing physical properties of its esterified amino acid and its codon–anticodon interaction. The sequence of the T-stem of each tRNA compensates for the differential effect of the esterified amino acid on the binding and release of EF-Tu during decoding. The sequence and modifications in the anticodon loop and core of tRNA impact the codon–anticodon strength and the ability of the tRNA to bend during codon recognition. These discoveries impact the design of tRNAs for the efficient and accurate incorporation of unnatural amino acids into proteins using bacterial translation systems.

Original languageEnglish (US)
Pages (from-to)138-145
Number of pages8
JournalCurrent Opinion in Chemical Biology
Volume46
DOIs
StatePublished - Oct 2018

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Transfer RNA
Ribosomes
Tuning
Amino Acids
Peptide Elongation Factor Tu
Anticodon
Bacterial Proteins
Codon
Decoding
Physical properties

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

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Evolutionary tuning impacts the design of bacterial tRNAs for the incorporation of unnatural amino acids by ribosomes. / Uhlenbeck, Olke C.; Schrader, Jared M.

In: Current Opinion in Chemical Biology, Vol. 46, 10.2018, p. 138-145.

Research output: Contribution to journalReview article

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