Ex vivo transduction of microvascular free flaps for localized peptide delivery

Joseph V. Michaels, Michael Dobryansky, Robert D. Galiano, Daniel J. Ceradini, Robert Bonillas, Deirdre Jones, Natalie Seiser, Jamie P. Levine, Geoffrey C. Gurtner*, Joseph M. Serletti, Kirit A. Bhatt

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Gene therapy is a promising modality for the treatment of soft tissue malignancies. Our laboratory has developed a novel technique of gene transfer using microvascular free flaps that addresses many of the current barriers preventing gene therapy from achieving widespread clinical use. Our previous work has demonstrated our ability to transduce free flaps with an adenovirus encoding the reporter gene lacZ. In this current study, we show that microvascular free flaps can be transduced with an adenovirus encoding the angiogenesis inhibitor endostatin with high levels of local flap expression. These transduced free flaps were able to serve as "biologic pumps" and were able to secrete endostatin into the serum as demonstrated by enzyme-linked immunosorbent assay. This form of "biologic brachytherapy" could provide a novel approach for the continuous delivery of therapeutic genes to a localized area while avoiding many of the practical obstacles currently limiting gene therapy.

Original languageEnglish (US)
Pages (from-to)581-584
Number of pages4
JournalAnnals of plastic surgery
Volume52
Issue number6
DOIs
StatePublished - Jun 2004

ASJC Scopus subject areas

  • Surgery

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