Exercise-induced promotion of hippocampal cell proliferation requires β-endorphin

M. Koeh*, P. Meerlo, D. Gonzales, A. Rontal, F. W. Turek, D. N. Abrous

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Adult hippocampal neurogenesis is influenced by a variety of stimuli, including exercise, but the mechanisms by which running affects neurogenesis are not yet fully understood. Because β-endorphin, which is released in response to exercise, increases cell proliferation in vitro, we hypothesized that it could exert a similar effect in vivo and mediate the stimulatory effects of running on neurogenesis. We thus analyzed the effects of voluntary wheel-running on adult neurogenesis (proliferation, differentiation, survival/death) in wild-type and β-endorphin-deficient mice. In wild-type mice, exercise promoted cell proliferation evaluated by sacrificing animals 24 h after the last 5-bromo-2́-deoxyuridine (BrdU) pulse and by using endogenous cell cycle markers (Ki67 and pH3). This was accompanied by an increased survival of 4-wk-old BrdU-labeled cells, leading to a net increase of neurogenesis. β-Endorphin deficiency had no effect in sedentary mice, but it completely blocked the running-induced increase in cell proliferation; this blockade was accompanied by an increased survival of 4-wk-old cells and a decreased cell death. Altogether, adult neurogenesis was increased in response to exercise in knockout mice. We conclude that β-endorphin released during running is a key factor for exercise-induced cell proliferation and that a homeostatic balance may regulate the final number of new neurons.

Original languageEnglish (US)
Pages (from-to)2253-2262
Number of pages10
JournalFASEB Journal
Volume22
Issue number7
DOIs
StatePublished - Jul 2008

Keywords

  • Adult neurogenesis
  • Cell survival
  • Homeostatic balance
  • Opioids
  • Running

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Exercise-induced promotion of hippocampal cell proliferation requires β-endorphin'. Together they form a unique fingerprint.

Cite this