Expanding the metabolic engineering toolbox: more options to engineer cells

Keith E. Tyo*, Hal S. Alper, Gregory N. Stephanopoulos

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

183 Scopus citations


Metabolic engineering exploits an integrated, systems-level approach for optimizing a desired cellular property or phenotype; and great strides have been made within this scope and context during the past fifteen years. However, due to limitations in the concepts and techniques, these have relied on a focused, pathway-oriented view. Recent advances in 'omics' technologies and computational systems biology have brought the foundational systems approach of metabolic engineering into focus. At the same time, protein engineering and synthetic biology have expanded the breadth and precision of the methods available to metabolic engineers to improve strain properties. Examples are presented that illustrate this broader perspective of tools and concepts, including a recent approach for global transcriptional machinery engineering (gTME), which has demonstrated the ability to elicit multigenic transcriptional changes that have improved phenotypes compared with single-gene perturbations.

Original languageEnglish (US)
Pages (from-to)132-137
Number of pages6
JournalTrends in biotechnology
Issue number3
StatePublished - Mar 2007

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


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