Expansions of clonal and oligoclonal T cells in B-cell chronic lymphocytic leukemia are primarily restricted to the CD3+CD8+ T-cell population

Charles Lewis Goolsby*, Miroslaw Kuchnio, William G. Finn, LoAnn Charlotte Peterson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


B-cell chronic lymphocytic leukemia (B-CLL) is characterized by the accumulation of mature-appearing clonal B cells exhibiting coexpression of CD5 and CD23. In addition to the accumulation of neoplastic B cells, numerous T-cell abnormalities also occur in B-CLL patients. In this study, the presence, and distribution within the T-cell subsets, of clonal/oligoclonal T cells was studied. Multicolor flow cytometric techniques were employed using combinations of anti-CD3, anti-CD4, and anti-CD8 antibodies coupled with antibodies specific for V(α) and V(β) T-cell receptor (TCR) epitopes. Molecular studies of TCR gene sequences were done to confirm the presence of clonal/oligoclonal T-cell populations. In the flow cytometric studies, examination of V(α)/V(β) expression found evidence of clonal/oligoclonal expansion in 9 of 19 patients studied. In eight of the nine patients, the expansions were restricted to the CD3+CD8+ cell population. Molecular analyses were performed in 16 patients, 12 of whom showed a clonal or oligoclonal pattern. Of the four patients who were negative in the molecular analyses, all demonstrated flow cytometric evidence of clonal/oligoclonal expansions. Thus, when the flow cytometric and molecular analyses were considered together, all 16 patients for whom parallel analyses were done showed evidence of clonal/oligoclonal expansions. These results confirm previous work demonstrating that the majority of B-CLL patients harbor clonal/oligoclonal expansions within the T-cell population. Additionally, based on the relative numbers of cells expressing specific V(α) or V(β) epitopes, these results show that these expansions occur primarily within the CD3+CD8+ T-cell population. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)188-195
Number of pages8
JournalCommunications in Clinical Cytometry
Issue number3
StatePublished - Jun 15 2000


  • B-CLL
  • Clonal
  • Oligoclonal
  • Suppressor cells
  • T cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology


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