@article{42b25e9b7d674c148733ebfa95a3ae0c,
title = "Experiential input alters the phosphorylation of specific proteins in brain membranes",
abstract = "The effects of a training experience that involves foot shock on the endogenous phosphorylation of membrane-bound proteins from brain were studied. Crude membrane fractions were prepared from the cerebral cortex and neostriatum of animals that had been sacrificed by quick freezing. In vitro incubation of the membranes with gamma-3 2P-ATP, followed by SDS-gel electrophoresis of the phosphorylated substrates, revealed that the phosphorylation of two protein components (designated F and H-I) increased in preparations from animals that were subjected to a training experience 24 hr prior to sacrifice. These effects were greater in preparations from the neostriatum than from the cerebral cortex, and were observed in experiments using both rats and mice. Although all trained animals showed a high phosphorylation of bands F and H-I, control animals showed a greater variability in the phosphorylation of these bands. The results indicate that the phosphorylation of specific proteins may play a mediatory role in the processing of experiential information.",
keywords = "Brain-membranes, Gamma-P-ATP, Protein phosphorylation in vitro, SDS-gel electrophoresis, Training experience",
author = "Ehrlich, {Yigal H.} and Rabjohns, {Ronald R.} and Aryeh Routtenberg",
note = "Funding Information: specified via the effects of cyclic AMP on the phosphorylation of specific proteins. The ability of cyclic AMP to produce rapid changes in the phosphorylation state of specific proteins in synaptic plasma membranes has been related to its postulated role in the formation of post synaptic potentials [12,25]. Such mechanisms may be involved in short-lived responses of the target cells to the evoking stimulus. In addition, phosphorylative modifications can play a role in long-term processes. Studies using the adrenal medulla [2,9] and cells grown in culture [23] as model systems have indicated that the transsynaptic induction of enzymes involved in the biosynthesis of neurotransmitters is mediated by phosphorylation mechanisms activated by cyclic AMP. Several lines of investigation suggest that protein phosphorylation may be similarly involved in mediating input-dependent processes within the brain. Electrical [28] as well as neurohumoral [29] stimulation of cerebral cortex slices have been shown to increase the phosphorylation of membrane-bound proteins. Two specific proteins whose phosphorylation is cyclic AMP-regulated were identified in synaptic membrane fractions from the rat cerebrum This study was supported by grants from the Alfred P. Sloan Foundation, N.S.F. Grant BMS-19,481 and NIH Grants NS HL 10768-02 and MH 2528 l-D2. The expert technical assistance of J. Ratliff and M. Jolly is acknowledged. Gratitude is expressed to Dr. E. G. Brunngraber from the Missouri Institute of Psychiatry for critical evaluation of the manuscript.",
year = "1977",
month = feb,
doi = "10.1016/0091-3057(77)90068-5",
language = "English (US)",
volume = "6",
pages = "169--174",
journal = "Pharmacology, Biochemistry and Behavior",
issn = "0091-3057",
publisher = "Elsevier Inc.",
number = "2",
}