Experiential input alters the phosphorylation of specific proteins in brain membranes

Yigal H. Ehrlich*, Ronald R. Rabjohns, Aryeh Routtenberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The effects of a training experience that involves foot shock on the endogenous phosphorylation of membrane-bound proteins from brain were studied. Crude membrane fractions were prepared from the cerebral cortex and neostriatum of animals that had been sacrificed by quick freezing. In vitro incubation of the membranes with gamma-3 2P-ATP, followed by SDS-gel electrophoresis of the phosphorylated substrates, revealed that the phosphorylation of two protein components (designated F and H-I) increased in preparations from animals that were subjected to a training experience 24 hr prior to sacrifice. These effects were greater in preparations from the neostriatum than from the cerebral cortex, and were observed in experiments using both rats and mice. Although all trained animals showed a high phosphorylation of bands F and H-I, control animals showed a greater variability in the phosphorylation of these bands. The results indicate that the phosphorylation of specific proteins may play a mediatory role in the processing of experiential information.

Original languageEnglish (US)
Pages (from-to)169-174
Number of pages6
JournalPharmacology, Biochemistry and Behavior
Issue number2
StatePublished - Feb 1977


  • Brain-membranes
  • Gamma-P-ATP
  • Protein phosphorylation in vitro
  • SDS-gel electrophoresis
  • Training experience

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience


Dive into the research topics of 'Experiential input alters the phosphorylation of specific proteins in brain membranes'. Together they form a unique fingerprint.

Cite this