TY - JOUR
T1 - Experimental autoimmune encephalomyelitis in mice
AU - Terry, Rachael L.
AU - Ifergan, Igal
AU - Miller, Stephen D.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2014.
PY - 2015
Y1 - 2015
N2 - Experimental autoimmune encephalitis (EAE), the animal model of multiple sclerosis (MS), has provided significant insight into the mechanisms that initiate and drive autoimmunity. Several central nervous system proteins and peptides have been used to induce disease, in a number of different mouse strains, to model the diverse clinical presentations of MS. In this chapter, we detail the materials and methods used to induce active and adoptive EAE. We focus on disease induction in the SJL/J, C57BL/6, and BALB/c mouse strains, using peptides derived from proteolipid protein, myelin basic protein, and myelin oligodendrocyte glycoprotein. We also include a protocol for the isolation of leukocytes from the spinal cord and brain for flow cytometric analysis.
AB - Experimental autoimmune encephalitis (EAE), the animal model of multiple sclerosis (MS), has provided significant insight into the mechanisms that initiate and drive autoimmunity. Several central nervous system proteins and peptides have been used to induce disease, in a number of different mouse strains, to model the diverse clinical presentations of MS. In this chapter, we detail the materials and methods used to induce active and adoptive EAE. We focus on disease induction in the SJL/J, C57BL/6, and BALB/c mouse strains, using peptides derived from proteolipid protein, myelin basic protein, and myelin oligodendrocyte glycoprotein. We also include a protocol for the isolation of leukocytes from the spinal cord and brain for flow cytometric analysis.
KW - Autoimmune disease
KW - CD4<sup>+</sup> T cells
KW - Experimental autoimmune encephalomyelitis
KW - Mouse model
KW - Multiple sclerosis
UR - http://www.scopus.com/inward/record.url?scp=84938834522&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938834522&partnerID=8YFLogxK
U2 - 10.1007/7651_2014_88
DO - 10.1007/7651_2014_88
M3 - Article
C2 - 25005074
AN - SCOPUS:84938834522
SN - 1064-3745
VL - 1304
SP - 145
EP - 160
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -