Experimental autoimmune encephalomyelitis in the mouse

Stephen D. Miller, William J. Karpus, Todd Scott Davidson

Research output: Contribution to journalReview article

120 Scopus citations


This unit details the materials and methods required for both active induction and adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. A protocol for isolating CNS-infiltrating lymphocytes in EAE mice is included. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains.

Original languageEnglish (US)
Pages (from-to)15.1.1-15.1.20
JournalCurrent Protocols in Immunology
Issue numberSUPPL. 88
StatePublished - Mar 19 2010


  • CFA
  • CNS
  • Central nervous system
  • Complete Freunds adjuvant
  • EAE
  • Experimental autoimmune encephalomyelitis
  • MBP
  • MOG
  • Myelin basic protein
  • Myelin oligodendrocyte glycoprotein
  • PLP
  • Proteolipid protein

ASJC Scopus subject areas

  • Immunology

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