Experiments in scanning luminescence x-ray microscopy

Chris J. Jacobsen*, Steve Lindaas, Vivian Oehler, Shawn P. Williams, Sue Wirick, Xiaodong Zhang, Shirong Guo, Ilan Spector

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Scanning luminescence x-ray microscopy is based on collecting visible light emission from a sample region illuminated by an x-ray microprobe. We have tested the resolution of the method using P31 phosphor grains, and have obtained luminescence images of dye-labelled polystyrene spheres and sodium salicylate crystals. However, we have observed no light emission from the dyes DAPI, ethidium bromide, Hoecst 33258, and rhodamine phalloidon. Present efforts are aimed at improving our understanding of the luminescence process so as to find appropriate dyes for imaging dye-labelled biological specimens.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages223-231
Number of pages9
ISBN (Print)0819409146
StatePublished - 1993
EventSoft X-Ray Microscopy - San Diego, CA, USA
Duration: Jul 19 1992Jul 21 1992

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1741
ISSN (Print)0277-786X

Other

OtherSoft X-Ray Microscopy
CitySan Diego, CA, USA
Period7/19/927/21/92

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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