Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs

Rui Yi; Yi Qin; Ian G. Macara; Bryan R. Cullen

doi:10.1101/gad.1158803

Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs

Rui Yi, Yi Qin, Ian G. Macara, Bryan R. Cullen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2013 Scopus citations

Abstract

MicroRNAs (miRNAs) are initially expressed as long transcripts that are processed in the nucleus to yield ∼65-nucleotide (nt) RNA hairpin intermediates, termed pre-miRNAs, that are exported to the cytoplasm for additional processing to yield mature, ∼22-nt miRNAs. Here, we demonstrate that human pre-miRNA nuclear export, and miRNA function, are dependent on Exportin-5. Exportin-5 can bind pre-miRNAs specifically in vitro, but only in the presence of the Ran-GTP cofactor. Short hairpin RNAs, artificial pre-miRNA analogs used to express small interfering RNAs, also depend on Exportin-5 for nuclear export. Together, these findings define an additional cellular cofactor required for miRNA biogenesis and function.

Original language	English (US)
Pages (from-to)	3011-3016
Number of pages	6
Journal	Genes and Development
Volume	17
Issue number	24
DOIs	https://doi.org/10.1101/gad.1158803
State	Published - Dec 15 2003
Externally published	Yes

Keywords

MicroRNA
Nuclear export
RNA interference
siRNA

ASJC Scopus subject areas

Genetics
Developmental Biology

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AU - Yi, Rui

AU - Qin, Yi

AU - Macara, Ian G.

AU - Cullen, Bryan R.

PY - 2003/12/15

Y1 - 2003/12/15

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AB - MicroRNAs (miRNAs) are initially expressed as long transcripts that are processed in the nucleus to yield ∼65-nucleotide (nt) RNA hairpin intermediates, termed pre-miRNAs, that are exported to the cytoplasm for additional processing to yield mature, ∼22-nt miRNAs. Here, we demonstrate that human pre-miRNA nuclear export, and miRNA function, are dependent on Exportin-5. Exportin-5 can bind pre-miRNAs specifically in vitro, but only in the presence of the Ran-GTP cofactor. Short hairpin RNAs, artificial pre-miRNA analogs used to express small interfering RNAs, also depend on Exportin-5 for nuclear export. Together, these findings define an additional cellular cofactor required for miRNA biogenesis and function.

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KW - Nuclear export

KW - RNA interference

KW - siRNA

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Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs

Abstract

Keywords

ASJC Scopus subject areas

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