Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs

Rui Yi, Yi Qin, Ian G. Macara, Bryan R. Cullen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2013 Scopus citations

Abstract

MicroRNAs (miRNAs) are initially expressed as long transcripts that are processed in the nucleus to yield ∼65-nucleotide (nt) RNA hairpin intermediates, termed pre-miRNAs, that are exported to the cytoplasm for additional processing to yield mature, ∼22-nt miRNAs. Here, we demonstrate that human pre-miRNA nuclear export, and miRNA function, are dependent on Exportin-5. Exportin-5 can bind pre-miRNAs specifically in vitro, but only in the presence of the Ran-GTP cofactor. Short hairpin RNAs, artificial pre-miRNA analogs used to express small interfering RNAs, also depend on Exportin-5 for nuclear export. Together, these findings define an additional cellular cofactor required for miRNA biogenesis and function.

Original languageEnglish (US)
Pages (from-to)3011-3016
Number of pages6
JournalGenes and Development
Volume17
Issue number24
DOIs
StatePublished - Dec 15 2003
Externally publishedYes

Keywords

  • MicroRNA
  • Nuclear export
  • RNA interference
  • siRNA

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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