Exposure to 4,4′-DDE in visceral adipose tissue and weight loss in adolescents from the Teen-LABS cohort

Bruna Rubbo; Zhenjiang Li; Phum Tachachartvanich; Brittney O. Baumert; Hongxu Wang; Shudi Pan; Sarah Rock; Justin R. Ryder; Todd Jenkins; Stephanie Sisley; Xiangping Lin; Scott Bartell; Thomas H. Inge; Stavra Xanthakos; Brooklynn McNeil; Anna R. Robuck; Michele A. La Merrill; Douglas I. Walker; David V. Conti; Rob McConnell; Sandrah P. Eckel; Lida Chatzi

doi:10.1002/oby.24009

Exposure to 4,4′-DDE in visceral adipose tissue and weight loss in adolescents from the Teen-LABS cohort

Bruna Rubbo, Zhenjiang Li, Phum Tachachartvanich, Brittney O. Baumert, Hongxu Wang, Shudi Pan, Sarah Rock, Justin R. Ryder, Todd Jenkins, Stephanie Sisley, Xiangping Lin, Scott Bartell, Thomas H. Inge, Stavra Xanthakos, Brooklynn McNeil, Anna R. Robuck, Michele A. La Merrill, Douglas I. Walker, David V. Conti, Rob McConnellSandrah P. Eckel, Lida Chatzi^*

^*Corresponding author for this work

Surgery

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Objective: Dichlorodiphenyldichloroethylene (DDE), an obesogen accumulating in adipose tissue, is released into circulation with weight loss, although its impact is underexplored among adolescents. We tested the association using an integrative translational approach of epidemiological analysis among adolescents with obesity and in vitro measures exploring the impact of DDE on adipogenesis via preadipocytes. Methods: We included 63 participants from the Teen-Longitudinal Assessment of Bariatric Surgery (Teen-LABS) cohort. We assessed 4,4′-DDE in visceral adipose tissue at surgery and BMI and waist circumference at surgery and 0.5, 1, 3, and 5 years after. We conducted longitudinal analysis to estimate the interaction on weight loss between DDE and time since surgery. In vitro analysis quantified adipogenic differentiation in commercial human preadipocytes exposed to 4,4′-DDE via fluorescent staining and imaging. Results: A dose–response relationship was observed, with the low-exposure group having a greater reduction in BMI during the first year compared to higher-exposure groups and showing smaller regains compared to higher-exposure groups after the first year. In vitro analysis of preadipocytes treated with 4,4′-DDE during adipogenic differentiation for 12 days showed a concentration-dependent increase in lipid accumulation. Conclusions: DDE could contribute to weight trajectory among adolescents undergoing bariatric surgery, potentially mediated via promoted adipogenesis in preadipocytes.

Original language	English (US)
Pages (from-to)	1023-1032
Number of pages	10
Journal	Obesity
Volume	32
Issue number	5
DOIs	https://doi.org/10.1002/oby.24009
State	Published - May 2024

Funding

This work was funded by the grant R01ES030364, awarded by the National Institute of Environmental Health Sciences (NIEHS) to Prof. Chatzi. The Teen‐LABS consortium is supported by cooperative agreements with the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) through grants for a clinical coordinating center (UM1DK072493; Inge) and the Data Coordinating Center (UM1DK095710; Xie). This work is not a product of the US Government or the US Environmental Protection Agency (EPA), and the author/editor/speaker is not doing this work in any governmental capacity. The views expressed are those of the author/editor/speaker only and do not necessarily represent those of the United States or the US EPA.

ASJC Scopus subject areas

Medicine (miscellaneous)
Endocrinology, Diabetes and Metabolism
Endocrinology
Nutrition and Dietetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/oby.24009

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Rubbo, B., Li, Z., Tachachartvanich, P., Baumert, B. O., Wang, H., Pan, S., Rock, S., Ryder, J. R., Jenkins, T., Sisley, S., Lin, X., Bartell, S., Inge, T. H., Xanthakos, S., McNeil, B., Robuck, A. R., La Merrill, M. A., Walker, D. I., Conti, D. V., ... Chatzi, L. (2024). Exposure to 4,4′-DDE in visceral adipose tissue and weight loss in adolescents from the Teen-LABS cohort. Obesity, 32(5), 1023-1032. https://doi.org/10.1002/oby.24009

@article{cb71d0adb31848168b4c20bc8b0c3d59,

title = "Exposure to 4,4′-DDE in visceral adipose tissue and weight loss in adolescents from the Teen-LABS cohort",

abstract = "Objective: Dichlorodiphenyldichloroethylene (DDE), an obesogen accumulating in adipose tissue, is released into circulation with weight loss, although its impact is underexplored among adolescents. We tested the association using an integrative translational approach of epidemiological analysis among adolescents with obesity and in vitro measures exploring the impact of DDE on adipogenesis via preadipocytes. Methods: We included 63 participants from the Teen-Longitudinal Assessment of Bariatric Surgery (Teen-LABS) cohort. We assessed 4,4′-DDE in visceral adipose tissue at surgery and BMI and waist circumference at surgery and 0.5, 1, 3, and 5 years after. We conducted longitudinal analysis to estimate the interaction on weight loss between DDE and time since surgery. In vitro analysis quantified adipogenic differentiation in commercial human preadipocytes exposed to 4,4′-DDE via fluorescent staining and imaging. Results: A dose–response relationship was observed, with the low-exposure group having a greater reduction in BMI during the first year compared to higher-exposure groups and showing smaller regains compared to higher-exposure groups after the first year. In vitro analysis of preadipocytes treated with 4,4′-DDE during adipogenic differentiation for 12 days showed a concentration-dependent increase in lipid accumulation. Conclusions: DDE could contribute to weight trajectory among adolescents undergoing bariatric surgery, potentially mediated via promoted adipogenesis in preadipocytes.",

author = "Bruna Rubbo and Zhenjiang Li and Phum Tachachartvanich and Baumert, {Brittney O.} and Hongxu Wang and Shudi Pan and Sarah Rock and Ryder, {Justin R.} and Todd Jenkins and Stephanie Sisley and Xiangping Lin and Scott Bartell and Inge, {Thomas H.} and Stavra Xanthakos and Brooklynn McNeil and Robuck, {Anna R.} and {La Merrill}, {Michele A.} and Walker, {Douglas I.} and Conti, {David V.} and Rob McConnell and Eckel, {Sandrah P.} and Lida Chatzi",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors. Obesity published by Wiley Periodicals LLC on behalf of The Obesity Society.",

year = "2024",

month = may,

doi = "10.1002/oby.24009",

language = "English (US)",

volume = "32",

pages = "1023--1032",

journal = "Obesity",

issn = "1930-7381",

publisher = "John Wiley and Sons Inc.",

number = "5",

}

Rubbo, B, Li, Z, Tachachartvanich, P, Baumert, BO, Wang, H, Pan, S, Rock, S, Ryder, JR, Jenkins, T, Sisley, S, Lin, X, Bartell, S, Inge, TH, Xanthakos, S, McNeil, B, Robuck, AR, La Merrill, MA, Walker, DI, Conti, DV, McConnell, R, Eckel, SP & Chatzi, L 2024, 'Exposure to 4,4′-DDE in visceral adipose tissue and weight loss in adolescents from the Teen-LABS cohort', Obesity, vol. 32, no. 5, pp. 1023-1032. https://doi.org/10.1002/oby.24009

TY - JOUR

T1 - Exposure to 4,4′-DDE in visceral adipose tissue and weight loss in adolescents from the Teen-LABS cohort

AU - Rubbo, Bruna

AU - Li, Zhenjiang

AU - Tachachartvanich, Phum

AU - Baumert, Brittney O.

AU - Wang, Hongxu

AU - Pan, Shudi

AU - Rock, Sarah

AU - Ryder, Justin R.

AU - Jenkins, Todd

AU - Sisley, Stephanie

AU - Lin, Xiangping

AU - Bartell, Scott

AU - Inge, Thomas H.

AU - Xanthakos, Stavra

AU - McNeil, Brooklynn

AU - Robuck, Anna R.

AU - La Merrill, Michele A.

AU - Walker, Douglas I.

AU - Conti, David V.

AU - McConnell, Rob

AU - Eckel, Sandrah P.

AU - Chatzi, Lida

PY - 2024/5

Y1 - 2024/5

N2 - Objective: Dichlorodiphenyldichloroethylene (DDE), an obesogen accumulating in adipose tissue, is released into circulation with weight loss, although its impact is underexplored among adolescents. We tested the association using an integrative translational approach of epidemiological analysis among adolescents with obesity and in vitro measures exploring the impact of DDE on adipogenesis via preadipocytes. Methods: We included 63 participants from the Teen-Longitudinal Assessment of Bariatric Surgery (Teen-LABS) cohort. We assessed 4,4′-DDE in visceral adipose tissue at surgery and BMI and waist circumference at surgery and 0.5, 1, 3, and 5 years after. We conducted longitudinal analysis to estimate the interaction on weight loss between DDE and time since surgery. In vitro analysis quantified adipogenic differentiation in commercial human preadipocytes exposed to 4,4′-DDE via fluorescent staining and imaging. Results: A dose–response relationship was observed, with the low-exposure group having a greater reduction in BMI during the first year compared to higher-exposure groups and showing smaller regains compared to higher-exposure groups after the first year. In vitro analysis of preadipocytes treated with 4,4′-DDE during adipogenic differentiation for 12 days showed a concentration-dependent increase in lipid accumulation. Conclusions: DDE could contribute to weight trajectory among adolescents undergoing bariatric surgery, potentially mediated via promoted adipogenesis in preadipocytes.

AB - Objective: Dichlorodiphenyldichloroethylene (DDE), an obesogen accumulating in adipose tissue, is released into circulation with weight loss, although its impact is underexplored among adolescents. We tested the association using an integrative translational approach of epidemiological analysis among adolescents with obesity and in vitro measures exploring the impact of DDE on adipogenesis via preadipocytes. Methods: We included 63 participants from the Teen-Longitudinal Assessment of Bariatric Surgery (Teen-LABS) cohort. We assessed 4,4′-DDE in visceral adipose tissue at surgery and BMI and waist circumference at surgery and 0.5, 1, 3, and 5 years after. We conducted longitudinal analysis to estimate the interaction on weight loss between DDE and time since surgery. In vitro analysis quantified adipogenic differentiation in commercial human preadipocytes exposed to 4,4′-DDE via fluorescent staining and imaging. Results: A dose–response relationship was observed, with the low-exposure group having a greater reduction in BMI during the first year compared to higher-exposure groups and showing smaller regains compared to higher-exposure groups after the first year. In vitro analysis of preadipocytes treated with 4,4′-DDE during adipogenic differentiation for 12 days showed a concentration-dependent increase in lipid accumulation. Conclusions: DDE could contribute to weight trajectory among adolescents undergoing bariatric surgery, potentially mediated via promoted adipogenesis in preadipocytes.

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JF - Obesity

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ER -

Exposure to 4,4′-DDE in visceral adipose tissue and weight loss in adolescents from the Teen-LABS cohort

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