Expression analysis of BACE2 in brain and peripheral tissues

Brian D. Bennett, Safura Babu-Khan, Richard Loeloff, Jean Claude Louis, Eileen Curran, Martin Citron, Robert Vassar*

*Corresponding author for this work

Research output: Contribution to journalArticle

237 Scopus citations

Abstract

Beta-site amyloid precursor protein cleaving enzyme (BACE) is a novel transmembrane aspartic protease that possesses all the known characteristics of the β-secretase involved in Alzheimer's disease (Vassar, R., Bennett, B. D., Babu-Khan, S., Kahn, S., Mendiaz, E. A., Denis, P., Teplow, D. B., Ross, S., Amarante, P., Loeloff, R., Luo, Y., Fisher, S., Fuller, J., Edenson, S., Lile, J., Jarosinski, M. A., Biere, A. L., Curran, E., Burgess, T., Louis, J.-C., Collins, F., Treanor, J., Rogers, G., and Citron, M. (1999) Science 286, 735-741). We have analyzed the sequence and expression pattern of a BACE homolog termed BACE2. BACE and BACE2 are unique among aspartic proteases in that they possess a carboxyl-terminal extension with a predicted transmembrane region and together they define a new family. Northern analysis reveals that BACE2 mRNA is expressed at low levels in most human peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach, and trachea. Human adult and fetal whole brain and most adult brain subregions express very low or undetectable levels of BACE2 mRNA. In addition, in situ hybridization of adult rat brain shows that BACE2 mRNA is expressed at very low levels in most brain regions. The very low or undetectable levels of BACE2 mRNA in the brain are not consistent with the expression pattern predicted for β-secretase.

Original languageEnglish (US)
Pages (from-to)20647-20651
Number of pages5
JournalJournal of Biological Chemistry
Volume275
Issue number27
DOIs
StatePublished - Jul 7 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Bennett, B. D., Babu-Khan, S., Loeloff, R., Louis, J. C., Curran, E., Citron, M., & Vassar, R. (2000). Expression analysis of BACE2 in brain and peripheral tissues. Journal of Biological Chemistry, 275(27), 20647-20651. https://doi.org/10.1074/jbc.M002688200