Expression analysis of MIR182 and its associated target genes in advanced ovarian carcinoma

Brian D. McMillen, Margarita M. Aponte, Zhaojian Liu, Irene B. Helenowski, Denise M. Scholtens, Barbara M. Buttin, Jian Jun Wei*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


BRCA1/BRCA2 mutations are common and the hallmarks of high-grade serous ovarian carcinoma. We found that MIR182, a negative BRCA1 regulator, is significantly overexpressed in high-grade serous ovarian carcinoma. To examine whether overexpression of MIR182 and its target genes, including BRCA1, HMGA2 (high-mobility group A2), FOXO3 and MTSS1, are associated with high-grade serous ovarian carcinoma tumor types and clinical outcome, we studied MIR182 by in situ hybridization and its target gene expression by immunohistochemistry in 117 cases of advanced ovarian cancer. We found that high-grade serous ovarian carcinoma had significantly higher MIR182 (P0.0003) and HMGA2 (P0.04) expression, and significantly lower BRCA1 (P0.0001) and FOXO3 (P0.001) expression than normal controls. MIR182 is significantly correlated with MTSS1 expression (r0.31; P0.001), whereas other target genes did not show a significant correlation with MIR182, indicating a complicated regulatory mechanisms of these genes in high-grade serous ovarian carcinoma. Among the examined MIR182 target genes, only HMGA2 was significantly associated with serous type carcinomas (P0.01), ascites (P0.01) and high death rate (P0.02). FOXO3 expression was associated with lower-stage disease (P0.04) and solid growth pattern (P0.03). MIR182 expression is significantly higher in high-grade serous ovarian carcinoma than in fallopian tubes.

Original languageEnglish (US)
Pages (from-to)1644-1653
Number of pages10
JournalModern Pathology
Issue number12
StatePublished - Dec 2012


  • BRCA1
  • HMGA2; MTSS1
  • miR-182
  • ovarian cancer

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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