Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome-associated coronavirus

Pui Ying Peggy Law, Yuet Man Liu, Hua Geng, Ka Ho Kwan, Mary Miu Yee Waye, Yuan Yuan Ho*

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in both nucleus and cytoplasm of mammalian cells. Functional study revealed that overexpression of SARS 8b induced DNA synthesis. Coexpression of SARS 8b and SARS 6, a previously characterized SARS-CoV accessory protein, did not elicit synergistic effects on DNA synthesis.

Original languageEnglish (US)
Pages (from-to)3643-3648
Number of pages6
JournalFEBS Letters
Volume580
Issue number15
DOIs
StatePublished - Jun 26 2006

Keywords

  • Coronavirus
  • SARS 8b
  • SARS ORF 8
  • Severe acute respiratory syndrome
  • X5

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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