Expression and localization of transforming growth factor-β receptors type I and type II in the rat ventral prostate during regression

Isaac Yi Kim, Han Jong Ahn, David J. Zelner, Linda Park, Julia A. Sensibar, Chung Lee*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

Of the three ubiquitously expressed transforming growth factor-β (TGFβ) receptors, only type I and type II receptors contain serine/threonine kinase activity and have a direct role in TGFβ signal transduction. In the prostate, it has been reported that the level of type III receptor expression increases transiently after castration. However, the relationship between the TGFβ signaling receptors, type I and type II, and androgen is currently unclear. Thus, in the present study, we made an initial attempt to elucidate the effect of androgen on type I and type II receptor expression in the rat ventral prostate by measuring the levels of messenger RNA (mRNA) and protein at specific time points after castration up to 10 days. Within 3 days after castration, an increase in type II receptor mRNA was observed in the prostate, and the level continued to rise until 7 days postcastration (~8- fold increase). Between days 7-10 postcastration, no significant change in the level of type II receptor mRNA was observed. Testosterone administration immediately after castration abolished the induction of type II receptor mRNA during the same 10-day period. Western blot analysis performed for type II receptor showed a similar result, in that the level of type II receptor protein increased ~5-fold by day 10 postcastration. In a similar manner to the expression of type II receptor mRNA, the level of type I receptor mRNA increased steadily until day 7 postcastration (~6-fold increase). Between days 7-10 postcastration, the level of type I receptor mRNA did not change significantly. As with type II receptor mRNA, the induction of type I receptor mRNA was suppressed when testosterone was administered immediately after castration. To localize the expression of TGFβ receptor type II, immunohistochemical studies were performed. The results of these studies demonstrated a preferential localization of type II receptor in the prostatic epithelial cells and an increased staining intensity for the receptor after castration. Taken together, these data indicate that TGFβ signaling receptors, type I and type II, are under negative androgenic regulation at the transcriptional level and that TGFβ may be an important regulator of a stromal-epithelial interaction in the rat ventral prostate.

Original languageEnglish (US)
Pages (from-to)107-115
Number of pages9
JournalMolecular Endocrinology
Volume10
Issue number1
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Fingerprint Dive into the research topics of 'Expression and localization of transforming growth factor-β receptors type I and type II in the rat ventral prostate during regression'. Together they form a unique fingerprint.

Cite this