Expression and polarity reversal of V-type H+-ATPase during the mineralization-demineralization cycle in Porcellio scaber sternal epithelial cells

Andreas Ziegler*, Dirk Weihrauch, Monica Hagedorn, David W. Towle, Reiner Bleher

*Corresponding author for this work

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The formation and resorption of CaCO3 by epithelial cell layers require epithelial transport of protons. We used the anterior sternal epithelium of the terrestrial isopod Porcellio scaber as a model to study the expression pattern and immunolocalization of a V-type H+-ATPase during the mineralization and demineralization of intermittent CaCO3 deposits. Semiquantitative RT-PCR revealed that the expression of the V-type H +-ATPase increases from non Ca2+-transporting control stages to the stages of CaCO3 deposit formation and resorption. In the Ca2+-transporting stages the expression was larger in the anterior than in the posterior sternal epithelium, which is not involved in deposit formation and transports just moderate amounts of CaCO3. Immunocytochemistry of the B-subunit of the V-type H+-ATPase in the anterior sternal epithelium reveals an increase in the abundance of the protein within the basolateral membrane, from undetectable to strong signals in the control stage to the stages of CaCO3 deposit formation, respectively. From the stage of CaCO3 deposit formation to that of CaCO 3 resorption the signal decreased within the basolateral plasma membrane and increased within the apical plasma membrane. For the first time the results indicate a contribution of a V-type H+-ATPase to CaCO 3 deposition and a reversal of its polarity from the basolateral to the apical plasma membrane compartment within the same cells.

Original languageEnglish (US)
Pages (from-to)1749-1756
Number of pages8
JournalJournal of Experimental Biology
Volume207
Issue number10
DOIs
StatePublished - Apr 1 2004

Keywords

  • Biomineralization
  • Calcium carbonate
  • Crustacea
  • Epithelial H transport
  • Epithelium
  • Isopoda
  • Porcellio scaber

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Molecular Biology
  • Animal Science and Zoology
  • Insect Science

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