Expression and potential role of dentin phosphophoryn (DPP) in mouse embryonic tissues involved in epithelial-mesenchymal interactions and branching morphogenesis

Keith Alvares, Yashpal S Kanwar, Arthur Veis*

*Corresponding author for this work

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Dentin sialophosphoprotein (DSPP) is synthesized in both mesenchyme and epithelium at varying stages of tooth development. At the tooth cap stage, corresponding to embryonic day (E) 13.5 of mouse embryonic life, the phosphophoryn (DPP) portion of DSPP was immunohistochemically localized to the enamel organ with intense staining of oral ectoderm but no expression in dental follicle mesenchyme. Surprisingly, DPP was also expressed in ureteric bud branches of embryonic metanephric kidney and alveolar epithelial buds of developing lung. Reverse transcriptase-polymerase chain reaction analysis verified the presence of DSPP mRNA with identical sequences in the tooth, lung, and kidney. The DSPP-1- mouse with ablated DPP expression in the teeth, also exhibited aberrant organogenesis in kidney and lung. In the kidney, malformed metanephric S-shaped bodies and increased mesenchymal apoptosis were observed. Inclusion of anti-DPP antibodies in organ culture of metanephroi, harvested from E13.5 wild-type mice, likewise resulted in altered ureteric bud morphogenesis, suggesting a role for DPP in epithelial-mesenchymal interactions in meristic tissues during embryonic development.

Original languageEnglish (US)
Pages (from-to)2980-2990
Number of pages11
JournalDevelopmental Dynamics
Volume235
Issue number11
DOIs
StatePublished - Nov 1 2006

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Keywords

  • BMP2
  • Dentin sialophosphoprotein
  • Development
  • Epithelial-mesenchymal interactions
  • Kidney ureteric buds
  • Lung epithelial alveolar buds
  • Phosphophoryn
  • Tooth

ASJC Scopus subject areas

  • Developmental Biology

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