Expression and production of bioactive human interleukin-18 in transgenic tobacco plants

Bin Zhang; Ying Hua Yang; Yong Ming Lin; Qing Rao; Guo Guang Zheng; Ke Fu Wu

doi:10.1023/A:1025638623770

Expression and production of bioactive human interleukin-18 in transgenic tobacco plants

Bin Zhang, Ying Hua Yang, Yong Ming Lin, Qing Rao, Guo Guang Zheng, Ke Fu Wu^*

^*Corresponding author for this work

Driskill Graduate Training Program in Life Sciences

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05 % of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN-γ on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g^-1 tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.

Original language	English (US)
Pages (from-to)	1629-1635
Number of pages	7
Journal	Biotechnology Letters
Volume	25
Issue number	19
DOIs	https://doi.org/10.1023/A:1025638623770
State	Published - Oct 2003

Keywords

Bioactivity
Human IL-18
Interleukin-18
Tobacco
Transgenic plants

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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@article{a8e6d8b13b2345fb8df1461b28b2aa35,

title = "Expression and production of bioactive human interleukin-18 in transgenic tobacco plants",

abstract = "The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05 % of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN-γ on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g-1 tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.",

keywords = "Bioactivity, Human IL-18, Interleukin-18, Tobacco, Transgenic plants",

author = "Bin Zhang and Yang, {Ying Hua} and Lin, {Yong Ming} and Qing Rao and Zheng, {Guo Guang} and Wu, {Ke Fu}",

note = "Funding Information: This work was supported by the Tianjin Science and Technology Development program of China (003119311). We thank Ms Li-Li An, Xiao-Tong Ma, Yi-Qi Geng, and Mrs Zhen-Yu Cao, Ge Li as well as Xiu-Jun Zhang for their technical help.",

year = "2003",

month = oct,

doi = "10.1023/A:1025638623770",

language = "English (US)",

volume = "25",

pages = "1629--1635",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "19",

}

TY - JOUR

T1 - Expression and production of bioactive human interleukin-18 in transgenic tobacco plants

AU - Zhang, Bin

AU - Yang, Ying Hua

AU - Lin, Yong Ming

AU - Rao, Qing

AU - Zheng, Guo Guang

AU - Wu, Ke Fu

N1 - Funding Information: This work was supported by the Tianjin Science and Technology Development program of China (003119311). We thank Ms Li-Li An, Xiao-Tong Ma, Yi-Qi Geng, and Mrs Zhen-Yu Cao, Ge Li as well as Xiu-Jun Zhang for their technical help.

PY - 2003/10

Y1 - 2003/10

N2 - The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05 % of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN-γ on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g-1 tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.

AB - The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05 % of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN-γ on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g-1 tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.

KW - Bioactivity

KW - Human IL-18

KW - Interleukin-18

KW - Tobacco

KW - Transgenic plants

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C2 - 14584919

AN - SCOPUS:0141638584

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EP - 1635

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

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