Expression and production of bioactive human interleukin-18 in transgenic tobacco plants

Bin Zhang, Ying Hua Yang, Yong Ming Lin, Qing Rao, Guo Guang Zheng, Ke Fu Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05 % of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN-γ on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g-1 tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.

Original languageEnglish (US)
Pages (from-to)1629-1635
Number of pages7
JournalBiotechnology Letters
Volume25
Issue number19
DOIs
StatePublished - Oct 2003

Keywords

  • Bioactivity
  • Human IL-18
  • Interleukin-18
  • Tobacco
  • Transgenic plants

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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