Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer

Xinfeng Chen, Liping Wang, Jinyan Liu, Lan Huang, Yang Li, Qun Gao, Xiaojuan Shi, Jieyao Li, Feng Li, Zhen Zhang, Song Zhao, Bin Zhang, Pierre van Der Bruggen, Yi Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Melanoma-associated antigen (MAGE)-A3 and MAGE-C2 are antigens encoded by cancer-germline genes, and have been recognized as potential prognostic biomarkers and attractive targets for immunotherapy in multiple types of cancer. The present study aimed to analyze the clinicopathological significance of MAGE-A3/C2 expression in non-small cell lung cancer (NSCLC). The association betweenMAGE-A3/C2mRNA and protein expression, and the pathological characteristics and overall survival of patients with NSCLC was analyzed. In addition, the functional role of MAGE-A3 in human NSCLC cell line A549 was examined in vitro. MAGE-A3/C2 mRNA expression was identified in 73% (151/206) and 53% (109/206) of patients with NSCLC, respectively. MAGE-A3/C2 protein expression was identified in 58% (44/76) and 53% (40/76) of NSCLC cases, respectively. MAGE-A3 mRNA expression was observed to be associated with smoking history, disease stage and lymph node metastasis. However, no association was identified between MAGE-C2 mRNA expression and the clinicopathological characteristics of patients with NSCLC. MAGE-A3/C2-positive patients had a poorer survival rate compared with MAGE-A3/C2-negative patients. Multivariate analysis identified that MAGE-A3 expression may serve as an independent marker of poor prognosis in patients with NSCLC. Downregulation of MAGE-A3 mRNA expression in A549 cells resulted in lower migration and colony formation rates, and a higher amount of epithelial marker and lower amount of mesenchymal marker expression compared with the control group. These results indicate that MAGE-A3 serves a role in NSCLC cell metastasis through the induction of epithelial-mesenchymal transition. In conclusion, MAGE-A3 may serve as a diagnostic and prognostic biomarker for patients with NSCLC, due to its association with tumor progression and poor clinical outcome.

Original languageEnglish (US)
Pages (from-to)1609-1618
Number of pages10
JournalOncology Letters
Volume13
Issue number3
DOIs
StatePublished - Mar 2017

Keywords

  • Cancer-germline genes
  • Epithelial-mesenchymal transition
  • Non-small cell lung cancer
  • Prognostic biomarker

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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