Expression and secretion of foreign proteins in yeast using the ADH1 promoter and 97 K killer toxin signal sequence

Seok Jong Hong, Hyen Sam Kang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Foreign proteins, endo-β-1,4-glucanase of Bacillus subtilis, preS1+S2 region of hepatitis B virus large surface antigen, human β2-adrenergic receptor (hβ2AR), and bovine growth hormone (bGH) were expressed in Saccharomyces cerevisiae and secreted into the medium. These proteins were expressed using the alcohol dehydrogenase I (ADH1) promoter of Saccharomyces cerevisiae and secreted by signal sequence of the 97 K killer toxin gene of double-stranded linear DNA plasmid (pGKL1) of S. cerevisiae. All these proteins underwent severe modifications; in particular, N-glycosylation in the case of endo-β-1,4-glucanasc, hβ2AR, and preS1+S2. Seventy four percent of the expressed endo-β-1,4-glucanase was secreted into the culture medium. Highly modified proteins were detected in the culture medium and in the cell. Expressed hβ2AR, which has seven transmembrane domains, remained in the cell. The degrees of secretion and modification and the states of proteins in the culture medium and in the cell were quite different. These results indicated that the nature of the protein has a critical role in its secretion and modifications.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalJournal of Biochemistry and Molecular Biology
Volume31
Issue number2
StatePublished - Mar 31 1998

Keywords

  • 97 K killer toxin signal sequence
  • ADH1 promoter
  • Modification
  • Secretion
  • Yeast

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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