Expression and subcellular localization of Ror tyrosine kinase receptors are developmentally regulated in cultured hippocampal neurons

Sabrina Paganoni, Adriana B Ferreira*

*Corresponding author for this work

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Ror1 and Ror2 are two novel receptor tyrosine kinases that have been implicated in neuronal differentiation in Caenorhabditis elegans. As a first step toward elucidating their role in the mammalian brain, we analyzed their expression and localization patterns in hippocampal neurons. Our results showed that both receptors are expressed from early stages of development and that their protein levels peak during periods of active synapse formation. Immunocytochemical analysis indicated that Rorl and Ror2 are highly concentrated in the growth cones of immature neurons and are present throughout the somatodendritic compartment of mature hippocampal cells. Further analysis indicated that they are present not only in the cell membrane but also in Triton- and saponin-insoluble fractions, suggesting that they may be associated with both the cytoskeleton and membrane-bound organelles. Taken collectively, our results suggest that Ror1 and Ror2 might play a role during early stages of development in mammalian central neurons.

Original languageEnglish (US)
Pages (from-to)429-440
Number of pages12
JournalJournal of Neuroscience Research
Volume73
Issue number4
DOIs
StatePublished - Aug 15 2003

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Keywords

  • Axonal elongation
  • Cytoskeleton
  • Growth cones
  • Receptor tyrosine kinases
  • Synaptogenesis

ASJC Scopus subject areas

  • Neuroscience(all)

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