Abstract
In this study, we report the complete cDNA sequence, genomic organization and expression pattern of the Caenorhabditis elegans ClC chloride channel gene, clh-6. Two different types of reporter gene fusions suggest that clh-6 expression is restricted to two gamma-aminobutyric acid-ergic RME neurons. These results are in striking contrast with the wide tissue distribution of messenger RNA for the related mammalian isoforms, ClC-6 and ClC-7. The restricted expression pattern of clh-6 provides a unique opportunity to study the biological function of a neuronal ClC chloride channel.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 177-180 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 299 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 23 2001 |
Funding
We thank Elizabeth Link and Cori Bargmann for helpful comments, Megan Olarte and Craig Short for technical assistance, and members of the Miller lab for help and suggestions. We also thank David Piston for help with confocal microscopy, Andrew Fire for providing expression vectors and Alan Coulson for cosmids. This work was supported by National Institutes of Health grants AR44506 (A.L.G.), NS26115 (D.M.M.) and DK58212 (A.L.G., D.M.M.) and a research fellowship grant from the National Kidney Foundation (L.B.). The clh-6 cDNA sequence was deposited in GenBank (accession number AF328908).
Keywords
- Caenorhabditis elegans
- ClC channels
- Gamma-aminobutyric acid-ergic neurons
- Green fluorescent protein fusions
ASJC Scopus subject areas
- General Neuroscience