Expression of a ClC chloride channel in Caenorhabditis elegans gamma-aminobutyric acid-ergic neurons

Laura Bianchi; David M. Miller; Alfred L. George

doi:10.1016/S0304-3940(01)01525-7

Expression of a ClC chloride channel in Caenorhabditis elegans gamma-aminobutyric acid-ergic neurons

Laura Bianchi, David M. Miller, Alfred L. George^*

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

In this study, we report the complete cDNA sequence, genomic organization and expression pattern of the Caenorhabditis elegans ClC chloride channel gene, clh-6. Two different types of reporter gene fusions suggest that clh-6 expression is restricted to two gamma-aminobutyric acid-ergic RME neurons. These results are in striking contrast with the wide tissue distribution of messenger RNA for the related mammalian isoforms, ClC-6 and ClC-7. The restricted expression pattern of clh-6 provides a unique opportunity to study the biological function of a neuronal ClC chloride channel.

Original language	English (US)
Pages (from-to)	177-180
Number of pages	4
Journal	Neuroscience Letters
Volume	299
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(01)01525-7
State	Published - Feb 23 2001

Keywords

Caenorhabditis elegans
ClC channels
Gamma-aminobutyric acid-ergic neurons
Green fluorescent protein fusions

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(01)01525-7

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title = "Expression of a ClC chloride channel in Caenorhabditis elegans gamma-aminobutyric acid-ergic neurons",

abstract = "In this study, we report the complete cDNA sequence, genomic organization and expression pattern of the Caenorhabditis elegans ClC chloride channel gene, clh-6. Two different types of reporter gene fusions suggest that clh-6 expression is restricted to two gamma-aminobutyric acid-ergic RME neurons. These results are in striking contrast with the wide tissue distribution of messenger RNA for the related mammalian isoforms, ClC-6 and ClC-7. The restricted expression pattern of clh-6 provides a unique opportunity to study the biological function of a neuronal ClC chloride channel.",

keywords = "Caenorhabditis elegans, ClC channels, Gamma-aminobutyric acid-ergic neurons, Green fluorescent protein fusions",

author = "Laura Bianchi and Miller, {David M.} and George, {Alfred L.}",

note = "Funding Information: We thank Elizabeth Link and Cori Bargmann for helpful comments, Megan Olarte and Craig Short for technical assistance, and members of the Miller lab for help and suggestions. We also thank David Piston for help with confocal microscopy, Andrew Fire for providing expression vectors and Alan Coulson for cosmids. This work was supported by National Institutes of Health grants AR44506 (A.L.G.), NS26115 (D.M.M.) and DK58212 (A.L.G., D.M.M.) and a research fellowship grant from the National Kidney Foundation (L.B.). The clh-6 cDNA sequence was deposited in GenBank (accession number AF328908).",

year = "2001",

month = feb,

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doi = "10.1016/S0304-3940(01)01525-7",

language = "English (US)",

volume = "299",

pages = "177--180",

journal = "Neuroscience Letters",

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TY - JOUR

T1 - Expression of a ClC chloride channel in Caenorhabditis elegans gamma-aminobutyric acid-ergic neurons

AU - Bianchi, Laura

AU - Miller, David M.

AU - George, Alfred L.

N1 - Funding Information: We thank Elizabeth Link and Cori Bargmann for helpful comments, Megan Olarte and Craig Short for technical assistance, and members of the Miller lab for help and suggestions. We also thank David Piston for help with confocal microscopy, Andrew Fire for providing expression vectors and Alan Coulson for cosmids. This work was supported by National Institutes of Health grants AR44506 (A.L.G.), NS26115 (D.M.M.) and DK58212 (A.L.G., D.M.M.) and a research fellowship grant from the National Kidney Foundation (L.B.). The clh-6 cDNA sequence was deposited in GenBank (accession number AF328908).

PY - 2001/2/23

Y1 - 2001/2/23

N2 - In this study, we report the complete cDNA sequence, genomic organization and expression pattern of the Caenorhabditis elegans ClC chloride channel gene, clh-6. Two different types of reporter gene fusions suggest that clh-6 expression is restricted to two gamma-aminobutyric acid-ergic RME neurons. These results are in striking contrast with the wide tissue distribution of messenger RNA for the related mammalian isoforms, ClC-6 and ClC-7. The restricted expression pattern of clh-6 provides a unique opportunity to study the biological function of a neuronal ClC chloride channel.

AB - In this study, we report the complete cDNA sequence, genomic organization and expression pattern of the Caenorhabditis elegans ClC chloride channel gene, clh-6. Two different types of reporter gene fusions suggest that clh-6 expression is restricted to two gamma-aminobutyric acid-ergic RME neurons. These results are in striking contrast with the wide tissue distribution of messenger RNA for the related mammalian isoforms, ClC-6 and ClC-7. The restricted expression pattern of clh-6 provides a unique opportunity to study the biological function of a neuronal ClC chloride channel.

KW - Caenorhabditis elegans

KW - ClC channels

KW - Gamma-aminobutyric acid-ergic neurons

KW - Green fluorescent protein fusions

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DO - 10.1016/S0304-3940(01)01525-7

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AN - SCOPUS:0035936684

VL - 299

SP - 177

EP - 180

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -

Expression of a ClC chloride channel in Caenorhabditis elegans gamma-aminobutyric acid-ergic neurons

Abstract

Keywords

ASJC Scopus subject areas

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