Expression of a homeobox gene in the chick wing bud following application of retinoic acid and grafts of polarizing region tissue

Guillermo Oliver, Eddy M. De Robertis, Lewis Wolpert, Cheryll Tickle

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

Homeobox gene XlHbox 1 is expressed in a mesodermal gradient in vertebrate forelimbs with maximal expression anteriorly and proximally and may encode positional values. In chick wing buds, anterior cells can be reprogrammed to form posterior structures by grafts of polarizing region tissue and by beads soaked in retinoic acid (RA), which is a good candidate for an endogenous morphogen. Applications of RA anteriorly or at the bud apex, treatments which produce duplicated digits or truncations respectively, substantially increase the extent of mesodermal XlHbox 1 expression. Polarizing region grafts that also produce additional digits lead to a moderate increase. The effects of RA application and the behaviour of transplanted tissue shwo that only anterior cells are competent to exress XlHbox 1 and that expression is cell autonomous. Ectodermal expression in wing buds is enhanced by RA but not by polarizing region grafts and ectoderm/mesoderm recombinations show that the mesoderm is irreversibly affected. The changes in mesodermal expression do not fit the predictions of the simple model that XlBHbox 1 encodes anterior positional values but are correlated with a series of novel malformations of the shoulder girdle which, in normal wing buds, is derived from cells expressing XlHbox 1.

Original languageEnglish (US)
Pages (from-to)3093-3099
Number of pages7
JournalEMBO Journal
Volume9
Issue number10
DOIs
StatePublished - 1990

Keywords

  • chick embryo
  • homeobox gene
  • limb development
  • polarizing region
  • retinoic acid

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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