Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA

Daniel P. Schachtman*, Julian I. Schroeder, William J. Lucas, Julie A. Anderson, Richard F Gaber

*Corresponding author for this work

Research output: Contribution to journalArticle

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Abstract

Inward-rectifying potassium channels located in the plasma membrane of higher plant and animal cells contribute to cellular homeostasis and excitability. The genes encoding this specific class of K+ channels have not been functionally identified. This report shows that a single messenger RNA transcript from the Arabidopsis thaliana KAT1 complementary DNA confers the functional expression of a hyperpolarization-activated K+ channel in Xenopus oocytes. The channels encoded by KAT1 are highly selective for K + over other monovalent cations, are blocked by tetraethylammonium and barium, and have a single-channel conductance of 28 ± 7 picosiemens with 118 millimolar K+ in the bathing solution. These functional characteristics, typical of inward-rectifying K+ channels in eukaryotic cells, demonstrate that KAT1 encodes an inward-rectifying K + channel.

Original languageEnglish (US)
Pages (from-to)1654-1658
Number of pages5
JournalScience
Volume258
Issue number5088
DOIs
StatePublished - Jan 1 1992

ASJC Scopus subject areas

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    Schachtman, D. P., Schroeder, J. I., Lucas, W. J., Anderson, J. A., & Gaber, R. F. (1992). Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA. Science, 258(5088), 1654-1658. https://doi.org/10.1126/science.8966547