Expression of annexin I in human pancreatic cancer and the influence of its down-regulation on biology of this cancer

Qian Liu*, Cheng Feng Wang, Zhuan Zhou, Hai Hu, Dong Bing Zhao, Xiao Guang Ni, Xiao Feng Bai, Ji Dong Gao, Yan Tao Tian, Ping Zhao

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

OBJECTIVE: To investigate the expression of annexin in human pancreatic cancer and to elucidate its role in oncogenesis of pancreatic cancer. METHODS: A pancreatic carcinoma cell line Suit-II with high-expression of annexin I gene was adopted. Three subtypes of annexin I -siRNA sequences and a non-related fragment were combined, and the eukaryotic expression vectors bearing siRNA fragments were constructed. Then they were transfected into pancreatic carcinoma cells to knock down the expression of annexin I by RNAi. After knocking down the expression of annexin I , the growth speed, cell cycling, morphological features and apoptosis of pancreatic carcinoma cells were examined by RT-PCR and MTT test. RESULTS: When the expression of annexin I was blocked, the growth speed of pancreatic carcinoma cells was significantly decreased, the morphological features were changed and pronounced apoptosis occurred. CONCLUSION: Annexin I can modulate pancreatic carcinoma cell cycle, promote the cell proliferation, increasingly stimulate the cell growth, and suppress the process of apoptosis in pancreatic carcinoma cells.

Original languageEnglish (US)
Pages (from-to)738-741
Number of pages4
JournalZhonghua zhong liu za zhi [Chinese journal of oncology]
Volume29
Issue number10
StatePublished - Oct 2007

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Expression of annexin I in human pancreatic cancer and the influence of its down-regulation on biology of this cancer'. Together they form a unique fingerprint.

  • Cite this

    Liu, Q., Wang, C. F., Zhou, Z., Hu, H., Zhao, D. B., Ni, X. G., Bai, X. F., Gao, J. D., Tian, Y. T., & Zhao, P. (2007). Expression of annexin I in human pancreatic cancer and the influence of its down-regulation on biology of this cancer. Zhonghua zhong liu za zhi [Chinese journal of oncology], 29(10), 738-741.