Expression of background potassium channels in rat DRG is cell-specific and down-regulated in a neuropathic pain model

Sarah L. Pollema-Mays, Maria Virginia Centeno, Crystle J. Ashford, Apkar Apkarian, Marco Martina*

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Neuropathic pain is associated with hyperexcitability of DRG neurons. Despite the importance of leakage potassium channels for neuronal excitability, little is known about their cell-specific expression in DRGs and possible modulation in neuropathic pain. Multiple leakage channels are expressed in DRG neurons, including TASK1, TASK3, TRESK, TRAAK, TWIK1, TREK1 and TREK2 but little is known about their distribution among different cell types. Our immunohistochemical studies show robust TWIK1 expression in large and medium size neurons, without overlap with TRPV1 or IB4 staining. TASK1 and TASK3, on the contrary, are selectively expressed in small cells; TASK1 expression closely overlaps TRPV1-positive cells, while TASK3 is expressed in TRPV1- and IB4-negative cells. We also studied mRNA expression of these channels in L4-L5 DRGs in control conditions and up to 4. weeks after spared nerve injury lesion. We found that TWIK1 expression is much higher than TASK1 and TASK3 and is strongly decreased 1, 2 and 4. weeks after neuropathic injury. TASK3 expression, on the other hand, decreases 1. week after surgery but reverts to baseline by 2. weeks; TASK1 shows no significant change at any time point. These data suggest an involvement of TWIK1 in the maintenance of the pain condition.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalMolecular and Cellular Neuroscience
Volume57
DOIs
StatePublished - Nov 1 2013

Fingerprint

Tandem Pore Domain Potassium Channels
Diagnosis-Related Groups
Neuralgia
Neurons
Potassium Channels
Wounds and Injuries
Maintenance
Staining and Labeling
Pain
Messenger RNA

Keywords

  • Immunohistochemistry
  • KCNK
  • Nerve injury
  • PCR
  • TWIK1

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

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title = "Expression of background potassium channels in rat DRG is cell-specific and down-regulated in a neuropathic pain model",
abstract = "Neuropathic pain is associated with hyperexcitability of DRG neurons. Despite the importance of leakage potassium channels for neuronal excitability, little is known about their cell-specific expression in DRGs and possible modulation in neuropathic pain. Multiple leakage channels are expressed in DRG neurons, including TASK1, TASK3, TRESK, TRAAK, TWIK1, TREK1 and TREK2 but little is known about their distribution among different cell types. Our immunohistochemical studies show robust TWIK1 expression in large and medium size neurons, without overlap with TRPV1 or IB4 staining. TASK1 and TASK3, on the contrary, are selectively expressed in small cells; TASK1 expression closely overlaps TRPV1-positive cells, while TASK3 is expressed in TRPV1- and IB4-negative cells. We also studied mRNA expression of these channels in L4-L5 DRGs in control conditions and up to 4. weeks after spared nerve injury lesion. We found that TWIK1 expression is much higher than TASK1 and TASK3 and is strongly decreased 1, 2 and 4. weeks after neuropathic injury. TASK3 expression, on the other hand, decreases 1. week after surgery but reverts to baseline by 2. weeks; TASK1 shows no significant change at any time point. These data suggest an involvement of TWIK1 in the maintenance of the pain condition.",
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Expression of background potassium channels in rat DRG is cell-specific and down-regulated in a neuropathic pain model. / Pollema-Mays, Sarah L.; Centeno, Maria Virginia; Ashford, Crystle J.; Apkarian, Apkar; Martina, Marco.

In: Molecular and Cellular Neuroscience, Vol. 57, 01.11.2013, p. 1-9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of background potassium channels in rat DRG is cell-specific and down-regulated in a neuropathic pain model

AU - Pollema-Mays, Sarah L.

AU - Centeno, Maria Virginia

AU - Ashford, Crystle J.

AU - Apkarian, Apkar

AU - Martina, Marco

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Neuropathic pain is associated with hyperexcitability of DRG neurons. Despite the importance of leakage potassium channels for neuronal excitability, little is known about their cell-specific expression in DRGs and possible modulation in neuropathic pain. Multiple leakage channels are expressed in DRG neurons, including TASK1, TASK3, TRESK, TRAAK, TWIK1, TREK1 and TREK2 but little is known about their distribution among different cell types. Our immunohistochemical studies show robust TWIK1 expression in large and medium size neurons, without overlap with TRPV1 or IB4 staining. TASK1 and TASK3, on the contrary, are selectively expressed in small cells; TASK1 expression closely overlaps TRPV1-positive cells, while TASK3 is expressed in TRPV1- and IB4-negative cells. We also studied mRNA expression of these channels in L4-L5 DRGs in control conditions and up to 4. weeks after spared nerve injury lesion. We found that TWIK1 expression is much higher than TASK1 and TASK3 and is strongly decreased 1, 2 and 4. weeks after neuropathic injury. TASK3 expression, on the other hand, decreases 1. week after surgery but reverts to baseline by 2. weeks; TASK1 shows no significant change at any time point. These data suggest an involvement of TWIK1 in the maintenance of the pain condition.

AB - Neuropathic pain is associated with hyperexcitability of DRG neurons. Despite the importance of leakage potassium channels for neuronal excitability, little is known about their cell-specific expression in DRGs and possible modulation in neuropathic pain. Multiple leakage channels are expressed in DRG neurons, including TASK1, TASK3, TRESK, TRAAK, TWIK1, TREK1 and TREK2 but little is known about their distribution among different cell types. Our immunohistochemical studies show robust TWIK1 expression in large and medium size neurons, without overlap with TRPV1 or IB4 staining. TASK1 and TASK3, on the contrary, are selectively expressed in small cells; TASK1 expression closely overlaps TRPV1-positive cells, while TASK3 is expressed in TRPV1- and IB4-negative cells. We also studied mRNA expression of these channels in L4-L5 DRGs in control conditions and up to 4. weeks after spared nerve injury lesion. We found that TWIK1 expression is much higher than TASK1 and TASK3 and is strongly decreased 1, 2 and 4. weeks after neuropathic injury. TASK3 expression, on the other hand, decreases 1. week after surgery but reverts to baseline by 2. weeks; TASK1 shows no significant change at any time point. These data suggest an involvement of TWIK1 in the maintenance of the pain condition.

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