Expression of bioactive human M-CSF soluble receptor in transgenic tobacco plants

Guo Guang Zheng*, Ying Hua Yang, Qing Rao, Yong Min Lin, Bin Zhang, Ke Fu Wu

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The cDNA encoding N-terminal three immunoglobin-like domains of human M-CSFR was linked to His-tag and endoplasmic reticulum retention sequence (KDEL) before being inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, by Agrobacterium tumefaciens-mediated transformation. The insertion and expression of target gene were confirmed by PCR, ELISA, and Western blot. The recombinant M-CSFsR reached a maximum expression level of 1.92% of total soluble protein in transgenic tobacco plant leaf tissues. The recombinant M-CSFsR could be purified through a one-step IMAC process and its bioactivity was confirmed by the inhibition of colony formation of J6-1 cells. The results suggested that we successfully expressed a high level of bioactive human M-CSFsR in tobacco plants.

Original languageEnglish (US)
Pages (from-to)367-373
Number of pages7
JournalProtein Expression and Purification
Volume46
Issue number2
DOIs
StatePublished - Apr 1 2006

Keywords

  • Bioactive
  • Macrophage colony-stimulating factor
  • Soluble receptor
  • Tobacco
  • Transgenic plants

ASJC Scopus subject areas

  • Biotechnology

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