Expression of chicken smooth muscle myosin light chain kinase in NIH 3T3 cells

Yuan Wang; Shu Yu Gui; D. M. Watterson

Expression of chicken smooth muscle myosin light chain kinase in NIH 3T3 cells

Yuan Wang^*, Shu Yu Gui, D. M. Watterson

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

Abstract

The myosin light chain kinase(MLCK) is important in regulating the smooth muscle contraction. The pBKrsv-MLCK was obtained by inserting MLCK cDNA into pBKrsv polylinker and transfected into NIH 3T3 cells. The results revealed that the transfected cells can express chicken MLCK identified by DNA-PCR, RT-PCR and Western blot. The expressed MLCK has high activity, thus providing the basis for further investigation of the signal transduction and regulation of smooth muscle contraction of MLCK.

Original language	English (US)
Pages (from-to)	161-162
Number of pages	2
Journal	Acta biochimica et biophysica Sinica
Volume	29
Issue number	2
State	Published - Dec 1 1997

Keywords

Expression
Myosin light chain kinase(MLCK)
NIH 3T3 cells
pBKrsv

ASJC Scopus subject areas

Biophysics
Biochemistry

Cite this

@article{821d4e79441a437b8791741da1996565,

title = "Expression of chicken smooth muscle myosin light chain kinase in NIH 3T3 cells",

abstract = "The myosin light chain kinase(MLCK) is important in regulating the smooth muscle contraction. The pBKrsv-MLCK was obtained by inserting MLCK cDNA into pBKrsv polylinker and transfected into NIH 3T3 cells. The results revealed that the transfected cells can express chicken MLCK identified by DNA-PCR, RT-PCR and Western blot. The expressed MLCK has high activity, thus providing the basis for further investigation of the signal transduction and regulation of smooth muscle contraction of MLCK.",

keywords = "Expression, Myosin light chain kinase(MLCK), NIH 3T3 cells, pBKrsv",

author = "Yuan Wang and Gui, {Shu Yu} and Watterson, {D. M.}",

year = "1997",

month = dec,

day = "1",

language = "English (US)",

volume = "29",

pages = "161--162",

journal = "Acta biochimica et biophysica Sinica",

issn = "0582-9879",

publisher = "Science Press",

number = "2",

}

TY - JOUR

T1 - Expression of chicken smooth muscle myosin light chain kinase in NIH 3T3 cells

AU - Wang, Yuan

AU - Gui, Shu Yu

AU - Watterson, D. M.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - The myosin light chain kinase(MLCK) is important in regulating the smooth muscle contraction. The pBKrsv-MLCK was obtained by inserting MLCK cDNA into pBKrsv polylinker and transfected into NIH 3T3 cells. The results revealed that the transfected cells can express chicken MLCK identified by DNA-PCR, RT-PCR and Western blot. The expressed MLCK has high activity, thus providing the basis for further investigation of the signal transduction and regulation of smooth muscle contraction of MLCK.

AB - The myosin light chain kinase(MLCK) is important in regulating the smooth muscle contraction. The pBKrsv-MLCK was obtained by inserting MLCK cDNA into pBKrsv polylinker and transfected into NIH 3T3 cells. The results revealed that the transfected cells can express chicken MLCK identified by DNA-PCR, RT-PCR and Western blot. The expressed MLCK has high activity, thus providing the basis for further investigation of the signal transduction and regulation of smooth muscle contraction of MLCK.

KW - Expression

KW - Myosin light chain kinase(MLCK)

KW - NIH 3T3 cells

KW - pBKrsv

UR - http://www.scopus.com/inward/record.url?scp=20444427647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444427647&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:20444427647

SN - 0582-9879

VL - 29

SP - 161

EP - 162

JO - Acta biochimica et biophysica Sinica

JF - Acta biochimica et biophysica Sinica

IS - 2

ER -

Expression of chicken smooth muscle myosin light chain kinase in NIH 3T3 cells

Abstract

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this