The myosin light chain kinase(MLCK) is important in regulating the smooth muscle contraction. The pBKrsv-MLCK was obtained by inserting MLCK cDNA into pBKrsv polylinker and transfected into NIH 3T3 cells. The results revealed that the transfected cells can express chicken MLCK identified by DNA-PCR, RT-PCR and Western blot. The expressed MLCK has high activity, thus providing the basis for further investigation of the signal transduction and regulation of smooth muscle contraction of MLCK.
|Original language||English (US)|
|Number of pages||2|
|Journal||Acta biochimica et biophysica Sinica|
|State||Published - Dec 1 1997|
- Myosin light chain kinase(MLCK)
- NIH 3T3 cells
ASJC Scopus subject areas