Expression of chicken smooth muscle myosin light chain kinase in NIH 3T3 cells

Yuan Wang*, Shu Yu Gui, D. M. Watterson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The myosin light chain kinase(MLCK) is important in regulating the smooth muscle contraction. The pBKrsv-MLCK was obtained by inserting MLCK cDNA into pBKrsv polylinker and transfected into NIH 3T3 cells. The results revealed that the transfected cells can express chicken MLCK identified by DNA-PCR, RT-PCR and Western blot. The expressed MLCK has high activity, thus providing the basis for further investigation of the signal transduction and regulation of smooth muscle contraction of MLCK.

Original languageEnglish (US)
Pages (from-to)161-162
Number of pages2
JournalActa biochimica et biophysica Sinica
Volume29
Issue number2
StatePublished - Dec 1 1997

Keywords

  • Expression
  • Myosin light chain kinase(MLCK)
  • NIH 3T3 cells
  • pBKrsv

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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