Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: dose-rate effects and protein synthesis requirements

Gayle E. Woloschak*, Paolo Felcher, Chin Mei Chang-Liu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Experiments were designed to examine the effects of radiation dose-rate and cycloheximide on expression of cytoskeletal and matrix elements in Syrian hamster embryo cells. Results here demonstrated little effect of dose-rate for fission-spectrum neutrons when comparing expression of α-tubulin or fibronectin genes. Effects of cycloheximide, however, revealed several findings: (a) Cycloheximide repressed accumulation of α-tubulin following exposure to high dose-rate neutrons or γ-rays. (b) Cycloheximide did not affect accumulation of mRNA for actin genes, (c) Cycloheximide abrogated the moderate induction of fibronectin-mRNA which occurred following exposure to γ-rays and high dose-rate neutrons. These results suggest a role for labile proteins in the maintenance of α-tubulin and fibronectin mRNA accumulation following exposure to ionizing radiation.

Original languageEnglish (US)
Pages (from-to)135-141
Number of pages7
JournalCancer Letters
Volume92
Issue number2
DOIs
StatePublished - Jun 8 1995

Keywords

  • DNA-damaging agents
  • Damage inducible genes
  • Ionizing radiation
  • Protein synthesis
  • Radiation dose-rate effects
  • Stress responses

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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