Abstract
Experiments were designed to examine the effects of radiation dose-rate and cycloheximide on expression of cytoskeletal and matrix elements in Syrian hamster embryo cells. Results here demonstrated little effect of dose-rate for fission-spectrum neutrons when comparing expression of α-tubulin or fibronectin genes. Effects of cycloheximide, however, revealed several findings: (a) Cycloheximide repressed accumulation of α-tubulin following exposure to high dose-rate neutrons or γ-rays. (b) Cycloheximide did not affect accumulation of mRNA for actin genes, (c) Cycloheximide abrogated the moderate induction of fibronectin-mRNA which occurred following exposure to γ-rays and high dose-rate neutrons. These results suggest a role for labile proteins in the maintenance of α-tubulin and fibronectin mRNA accumulation following exposure to ionizing radiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 135-141 |
| Number of pages | 7 |
| Journal | Cancer Letters |
| Volume | 92 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 8 1995 |
Funding
The authors wish to thank Mr Gordon Holmblad for help in irradiations, MS Kay Bexson for excellent secretarial assistance, and Dr Carol Giometti, Dr Frank Collar& and Dr Lydia Ling-Indeck for reviewing the manuscript prior to submission. This work was supported by the US Department of Energy, Office of Health and Environmental Research, under Contract No. W-31-109-ENG-38.
Keywords
- DNA-damaging agents
- Damage inducible genes
- Ionizing radiation
- Protein synthesis
- Radiation dose-rate effects
- Stress responses
ASJC Scopus subject areas
- Oncology
- Cancer Research
