Expression of functional chemokine receptors by rat cerebellar neurons

Samantha E. Gillard; Meiling Lu; Rose M. Mastracci; Richard J. Miller

doi:10.1016/S0165-5728(02)00005-X

Expression of functional chemokine receptors by rat cerebellar neurons

Samantha E. Gillard, Meiling Lu, Rose M. Mastracci, Richard J. Miller^*

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

In this study, we examined chemokine receptor expression and function in rat cerebellar neurons. Calcium imaging experiments demonstrated that a wide variety of chemokines elicited [Ca²⁺]_i transients in acutely isolated and cultured cerebellar Purkinje and granule neurons. In many cases, these chemokine responses were pertussis toxin (PTX) insensitive. In addition, chemokines activated the Ca²⁺ and cAMP-dependent transcription factor CREB and the extracellular response kinases ERK1/ERK2. Chemokines increased the survival of Purkinje neurons deprived of their trophic support. Thus, the presence of chemokine receptors and the signaling pathways they activate suggest that chemokines play a role in the control of cerebellar neuron survival and development and may mediate communication between the CNS and the immune system.

Original language	English (US)
Pages (from-to)	16-28
Number of pages	13
Journal	Journal of Neuroimmunology
Volume	124
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(02)00005-X
State	Published - 2002

Keywords

Calcium mobilization
Cerebellar neurons
Chemokines
Signal transduction
Viralkines

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/S0165-5728(02)00005-X

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@article{b0e7686fc88148d08fe621137dc6c7ba,

title = "Expression of functional chemokine receptors by rat cerebellar neurons",

abstract = "In this study, we examined chemokine receptor expression and function in rat cerebellar neurons. Calcium imaging experiments demonstrated that a wide variety of chemokines elicited [Ca2+]i transients in acutely isolated and cultured cerebellar Purkinje and granule neurons. In many cases, these chemokine responses were pertussis toxin (PTX) insensitive. In addition, chemokines activated the Ca2+ and cAMP-dependent transcription factor CREB and the extracellular response kinases ERK1/ERK2. Chemokines increased the survival of Purkinje neurons deprived of their trophic support. Thus, the presence of chemokine receptors and the signaling pathways they activate suggest that chemokines play a role in the control of cerebellar neuron survival and development and may mediate communication between the CNS and the immune system.",

keywords = "Calcium mobilization, Cerebellar neurons, Chemokines, Signal transduction, Viralkines",

author = "Gillard, {Samantha E.} and Meiling Lu and Mastracci, {Rose M.} and Miller, {Richard J.}",

note = "Funding Information: We gratefully thank Patrick Gray and Johnny Stine at ICOS corporation for the gift of chemokines and viral chemokines. This work was supported by the National Institutes of Health Grants DA-02121, MH-40165, NS-33826 and DA-13141 to R.J.M. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2002",

doi = "10.1016/S0165-5728(02)00005-X",

language = "English (US)",

volume = "124",

pages = "16--28",

journal = "Advances in Neuroimmunology",

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TY - JOUR

T1 - Expression of functional chemokine receptors by rat cerebellar neurons

AU - Gillard, Samantha E.

AU - Lu, Meiling

AU - Mastracci, Rose M.

AU - Miller, Richard J.

N1 - Funding Information: We gratefully thank Patrick Gray and Johnny Stine at ICOS corporation for the gift of chemokines and viral chemokines. This work was supported by the National Institutes of Health Grants DA-02121, MH-40165, NS-33826 and DA-13141 to R.J.M. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2002

Y1 - 2002

N2 - In this study, we examined chemokine receptor expression and function in rat cerebellar neurons. Calcium imaging experiments demonstrated that a wide variety of chemokines elicited [Ca2+]i transients in acutely isolated and cultured cerebellar Purkinje and granule neurons. In many cases, these chemokine responses were pertussis toxin (PTX) insensitive. In addition, chemokines activated the Ca2+ and cAMP-dependent transcription factor CREB and the extracellular response kinases ERK1/ERK2. Chemokines increased the survival of Purkinje neurons deprived of their trophic support. Thus, the presence of chemokine receptors and the signaling pathways they activate suggest that chemokines play a role in the control of cerebellar neuron survival and development and may mediate communication between the CNS and the immune system.

AB - In this study, we examined chemokine receptor expression and function in rat cerebellar neurons. Calcium imaging experiments demonstrated that a wide variety of chemokines elicited [Ca2+]i transients in acutely isolated and cultured cerebellar Purkinje and granule neurons. In many cases, these chemokine responses were pertussis toxin (PTX) insensitive. In addition, chemokines activated the Ca2+ and cAMP-dependent transcription factor CREB and the extracellular response kinases ERK1/ERK2. Chemokines increased the survival of Purkinje neurons deprived of their trophic support. Thus, the presence of chemokine receptors and the signaling pathways they activate suggest that chemokines play a role in the control of cerebellar neuron survival and development and may mediate communication between the CNS and the immune system.

KW - Calcium mobilization

KW - Cerebellar neurons

KW - Chemokines

KW - Signal transduction

KW - Viralkines

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U2 - 10.1016/S0165-5728(02)00005-X

DO - 10.1016/S0165-5728(02)00005-X

M3 - Article

C2 - 11958818

AN - SCOPUS:0036198825

SN - 0165-5728

VL - 124

SP - 16

EP - 28

JO - Advances in Neuroimmunology

JF - Advances in Neuroimmunology

IS - 1-2

ER -

Expression of functional chemokine receptors by rat cerebellar neurons

Abstract

Keywords

ASJC Scopus subject areas

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