Abstract
A clonal cell line of rat embryonic hippocampal origin (H19-7) has been examined for the expression of glucocorticoid receptors (GR) and mineralocorticoid receptors (MR). H19-7 cells grown at 33°C continue to divide, however when grown at 39°C in reduced levels of serum the cells undergo morphological differentiation and express neuronal properties. Immunocytochemistry demonstrated that H19-7 cells express both MR and GR when grown at either 33°C or 39°C. GR mRNA is readily detected in H19-7 cells by RNase protection assay. MR mRNA levels in H19-7 cells are too low to detect by RNase protection, but can be detected by RT-PCR. RT-PCR also demonstrated that H19-7 cells express more GR mRNA than primary hippocampal neurons. Since previous studies have shown that the level of MR mRNA is higher than that of GR mRNA in hippocampal neurons, these studies suggest that H19-7 cells represent hippocampal neurons immortalized at an early stage when the MR system is not yet fully differentiated.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 141-152 |
| Number of pages | 12 |
| Journal | Brain research |
| Volume | 726 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Jul 8 1996 |
Funding
The authors would like to thank Drs. J. Arriza, D. Batter, R. Evans, R. Miesfeld and K. Yamamoto for providing the cDNA clones, Dr. R. Harrison for providing the BuGR monoclonal antibody, Dr. Z. Krozowski for providing the MINREC4 polyclonal antibody and Dr. X. Breakefield for providing the retrovirus; also R. Giuliano for her technical help and D. Dean for his help with the RPAs. This study was supported by National Institutes of Health grants NS20832 (M.C.B.) and NS27587 (B.H.W.). C.M.B.-H. was supported by PHS AG00107 and DK07093 training grants.
Keywords
- Neuronal differentiation
- RT-PCR
- Steroid receptor
ASJC Scopus subject areas
- Clinical Neurology
- Molecular Biology
- General Neuroscience
- Developmental Biology