Expression of miR-124 inhibits growth of medulloblastoma cells

Joachim Silber, Rintaro Hashizume, Tristan Felix, Sujatmi Hariono, Mamie Yu, Mitchel S. Berger, Jason T. Huse, Scott R. Vandenberg, C. David James, J. Graeme Hodgson, Nalin Gupta*

*Corresponding author for this work

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Medulloblastoma is the most common malignant brain tumor in children, and a substantial number of patients die as a result of tumor progression. Overexpression of CDK6 is present in approximately one-third of medulloblastomas and is an independent poor prognostic marker for this disease. MicroRNA (miR)-124 inhibits expression of CDK6 and prevents proliferation of glioblastoma and medulloblastoma cells in vitro. We examined the effects of miR-124 overexpression on medulloblastoma cells both in vitro and in vivo and compared cell lines that have low and high CDK6 expression. MiR-124 overexpression inhibits the proliferation of medulloblastoma cells, and this effect is mediated mostly through the action of miR-124 upon CDK6. We further show that induced expression of miR-124 potently inhibits growth of medulloblastoma xenograft tumors in rodents. Further testing of miR-124 will help define the ultimate therapeutic potential of preclinical models of medulloblastoma in conjunction with various delivery strategies for treatment.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalNeuro-oncology
Volume15
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • CDK6
  • D425
  • medulloblastoma
  • miR-124
  • microRNA

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

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  • Cite this

    Silber, J., Hashizume, R., Felix, T., Hariono, S., Yu, M., Berger, M. S., Huse, J. T., Vandenberg, S. R., David James, C., Graeme Hodgson, J., & Gupta, N. (2013). Expression of miR-124 inhibits growth of medulloblastoma cells. Neuro-oncology, 15(1), 83-90. https://doi.org/10.1093/neuonc/nos281